Birgit K. Email & Phone Number

Bonn, North Rhine-Westphalia, Germany

• Compound (DMSO) spotting with Echo 550 with ultrasound, liquids are moved with sound from a source plate into an upside down destination plate, a variety of layouts are possible single or dose response.• Cell based assays (PBMC: peripheral blood mononuclear cells, THP1: human leukemic monocyte, SHSY5Y: human neuro blastoma cell) for Luminescence or Fluorescence.• Cell Voyager 8000, fully automated fluorescence microscope for 96, 384 or 1536 well plates,Live cell or fixated immune stained cells.• iPS cell differentiation (feeder independent pluripotent stem cell protocols) into BMEC (brain microvascular endothelial cells) to form a Blood Brain Barrier Model.• Measurement of TEER (Trans endothelial electrical Resistance) to test the integrity of this monolayer.• Immune Stain for tight junction marker: ZO-1, Occludin.• Co-culture with astrocytes and pericytes.• Cytokine measurements in cell culture supernatants with HTRF (homogeneous time resolved fluorescence) see Cisbio, plate reader paradigm or spectra max molecular devices, software softmax pro.

Cologne, North Rhine-Westphalia, Germany

Cancer research, cell based assays, primary cell culture, immunofluorescence staining, cytotoxicity, viability and apoptosis testing.