Brian Kinley Email & Phone Number

Austin, Texas, US

San Diego, CA, US

• Collaborate with experimental scientists to continuously improve efficiency and throughput by transitioning lab processes from manual into semi- and fully-automated workflows.
• Worked with with NGS, CEPA (Cloning, Expression, Purification and Analysis) and Assay Development teams to establish various NGS workflows, Automated Protein Purification and various assays on the Hamilton Liquid.
• Contributed to Encodia’s long-term laboratory automation strategy, identifying future equipment needs and collaborating with integration partners to deliver scalable solutions
• Create protocols/scripts to control automated lab equipment, Hamilton liquid handling platforms (STARS and Vantages), robotic arms, incubators, etc.
• Communicate the potential risks and tradeoffs of automation to scientific team members who will monitor outputs/results accordingly.
• Train users to troubleshoot/repair automated instruments and apply continuous improvement principles to increase uptime at a reduced cost

• Perform various chemical related wet lab experiments, electro-mechanical, and software troubleshooting on new synthesizer
• Provide input and design solutions to improve instrument usage and solve problematic areas of the new synthesizer instrument.
• Transfer oligonucleotide synthesis and processing setup and process.development documentation into workable manufacturing procedures.
• Provide input and design solutions to improve instrument usage and solveproblematic areas of the instrument.
• Optimized and customized automation platform to significantly reduce downstream cycle times and scientist labor.
• Refine software scripting routines to improve system performance.

Carlsbad, CA, US

• Subject matter expert for various Ionis automation platforms.
• Expertise in maintaining, troubleshooting and writing scripts and protocols with various automation schedulers as well as Beckman (FX and i7), Hamilton (Star) and Perkin Elmer (Janus) liquid handlers.
• Optimized and customized automation platform to significantly reduce downstream cycle times and scientist labor.
• Implemented new automation hardware and scripts to expand automation capabilities and support lead identification/screening, functional genomics, and cell based assays
• Trained and managed junior automation staff.
• Oversaw and refined Oligo Quality Control process utilizing Beckman liquid handlers and integrated automation systems.

• Manage Pfenex Automation equipment and staff.
• Evaluate, Develop, and Integrate new automation technologies.

• Subject matter expert for Pfenex’s various automation platforms.
• Expertise in maintaining, troubleshooting and writing protocols with various automation systems and Liquid Handlers including Tecan, Biomek, and Caliper systems.
• Developed and performed automated high throughput protocols for molecular biology and downstream purification utilizing Biosero Green Button Go, Tecan Evoware, Biomek Liquid Handling Software, and Perkin-Elmer Maestro.
• Optimized and customized automation platform to significantly reduce downstream cycle times and scientist labor.
• Managed and ordered lab supplies for all automation projects.

San Diego, CA, US

• Oversaw high throughput screening and automation efforts.
• Expertise in maintaining, troubleshooting and writing protocols with various automation systems including Agilent Benchbot, Biocel, and Bravo, as well as Molecular Devices colony pickers.
• Developed and performed automated high throughput enzyme assays leading to the discovery of multiple improved enzyme variants.
• Purified and characterized various enzyme variants demonstrating their improved properties.
• Optimized and customized automation platform to significantly increase screening throughput.
• Managed and ordered lab supplies for all HT screening efforts.

• Performed biochemical, phage display, and DNA link plate based screening assays as part of a kinase based drug discovery platform..
• Wrote protocols, used and maintained various robotic pipetting platforms to plate and perform HTS assays.
• Supervised small scale fermentation E. coli and managed inventory of frozen lysates.
• Maintained, and ran ABI qPCR machines to quantify results of kinase screening panel.
• Developed automation platform to significantly increase kinase screening throughput.

• Purified numerous protein types from E. coli and Baculovirus sources including kinases, nuclear receptors, and lipases in a high throughput setting using AKTA FPLC machines and other types of automation.
• Supervised technology transfer of purification platform to a large pharmaceutical company.
• Developed purification protocols and technology and purified over 300 bacterial proteins within a specified timeframe to transfer to a partner company under an N.I.H. grant.
• Oversaw maintenance and troubleshooting of AKTA FPLC machines and columns.

• Directed purification of several Proteases, including TEV protease and EK max primarily using AKTA FPLC machines, Explorers, and Purifiers.
• Performed antibody purifications.
• Executed sterile tissue culture techniques by maintaining several lines of mammalian cells.
• Utilized numerous types of resins in XK and Index columns. Packed, equilibrated, and maintained all columns.
• Successfully worked in a team atmosphere to package and aliquot purified enzymes.