Randy Bledsoe Email & Phone Number

Chapel Hill, Nc, Us

Chapel Hill, Nc, Us

Durham, North Carolina, Us

Downstream lab-scale process development and cGMP production support of proteins and mRNA entities.

Brentford, Middlesex, Gb

Searching available bioinformatics databases to analyze DNA and protein sequencesAnalyzing existing protein structures via PyMOL for domain determinations and structural comparisonsDesigning expression constructs for bacterial, mammalian or insect cell expression systemsUtilizing molecular biology techniques (PCR, subcloning, DNA amplification, DNA sequence verification, manipulation and purification) to generate target constructsUsing site-directed mutagenesis for optimizing expression constructs including engineering non-native disulfide bonds, incorporating mutations for increasing/decreasing ligand affinities, modifying glycosylation sites and removing problematic loops/sequencesEvaluating fusion partners and protein co-expression systemsOptimizing methods for E. coli expression including evaluation of media, temperature and expression duration Developing refolding protocols for difficult to obtain proteins and protein domains via size exclusion, rapid drop and dialysis methodsPurifying proteins and protein domains for use in assay development, antibody production, crystallography etc. using FPLC (ÄKTApurifier, ÄKTAxpress and ÄKTAavant) for affinity, ion exchange, hydrophobic interaction and size exclusion chromatographyCharacterizing and validating purified proteins (SDS-PAGE, Mass Spec analysis, protein concentration determination)

Master Of Science (Msc), Biochemistry

Bachelor Of Arts (B.A.), Chemistry