Lab automation and screening operations team lead.

Compound Logistics Automation

o Primary contributor in the validation and optimization of the Bionex Dual-Hive automation system for execution of Vividion's proteomic sample prep assay. Created an alternate workflow for troubleshooting the validation of the system by utilizing protein and peptide quantification assays instead of MS analysis to increase throughput of troubleshooting period by 300%.o Developed, optimized, and validated an automated ELISA protocol for application in weekly compound profiling for a lead target. Enabled scientists to increase throughput by 6 hours per week. o Developed, optimized, and validated a VWorks form and 9 protocols to enable fully automated compound plate production for the Platform & Proteomics group. Scripted with JavaScript to allow for combinations of inputs to be captured and used to drive the generation of a runset to produce plates in any desired plate format and dilution scheme. Projected to save 10 FTE hours per week.o Inclusive of all responsibilities as Research Associate I at Vividion Therapeutics.

o Execution of Vividion’s proprietary proteomic sample prep assay on a weekly basis to generate MS data enabling the assessment of compound target engagement proteome-wide for new screens and lead evaluation studies. Involved upstream workflow of converting study requests into associated mass-spec experiment ID(s), compound plates, and tracking of experiment progress through the 3-4 day sample prep protocol.o Led Vividion’s design, implementation, and validation of the Bionex Dual-Hive automation system. Involved concept design of a custom dispenser coupled with a vacuum filtration manifold to reduce plate-handling time by 60% for filtration steps during automated runs. Mitigated project challenges involving global supplier shortages and fluid timelines impacting finance decisions over the course of a year.o Developed, optimized, and validated Bravo protocols for all use cases within the Platform & Proteomics group and Biology department, enabling rapid execution of various compound dilution schemes, ELISA(s), and plate reformatting. Protocols were scripted with JavaScript to provide maximum end-user flexibility and ease of use through variable capture and decision trees.o Primary contributor in the management and execution of Vividion’s compound library stamp into mother plates and assay-ready compound plates for use in the proteomic sample prep assay for screening. Developed, optimized, and validated all Bravo protocols and liquid classes used in this process. o Trained end-users to transition assay development and production assays from manual execution to semi- or fully-automated execution to increase throughput and reliability of assay execution. Involved protocol development and validation on platforms like the Agilent Bravo, Formulatrix Mantis, BioTek EL 406, and Bionex Dual-Hive automation system.

o Operating and troubleshooting the GNF uHTS automation platform to execute weekly assays enabling SAR studies and multiple primary HTS screens in 1536-well biochemical and cell-based formats o Executing weekly radioligand binding assays in 384-well format to generate and analyze affinity binding data enabling SAR and selectivity studies of novel compoundso Performing tissue culture and transient transfections to produce and qualify membrane proteins used in various radioligand binding assayso Developing and validating a 96-well radioligand binding assay to screen novel compounds for selectivity binding data over receptor subtypeso Assisting in the assay development of multiple TR-FRET 1536-well cell-based assays for new targetso Contributing to various projects via method development for liquid handling and automationo Troubleshooting automated screens, liquid handlers, lab hardware, and peripheral device softwareo Writing optimized protocols, calibration, and maintenance for a range of liquid handlers o Management of lab inventory

o Expanded knowledge of assay development concepts and techniques and uHTS automation platform o Application of GNF/Kalypsys uHTS automation system for HTSo Application of Labcyte Echo for assay developmento Calibration, maintenance, and troubleshooting of various liquid handlers and readers through developing QC protocols with managero Provided support to scientists through developing liquid handling protocols for manual and automated settingso Managed lab inventory and coordinated lab workflow for uHTS department