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Highly skilled biochemist and molecular biologist with 20 years research experience in experimental design, execution, data analysis and troubleshooting, with a strong background on molecular biology and recombinant protein/antibody expression, purification, characterization, and biochemical analysis. Excellent leader in the biotech/biopharma company. Extensive experience in recombinant expression of monoclonal/bi-specific/tri-specific antibodies and proteins, from expression constructs design/generation, transfection /transformation to recombinant protein expression in mammalian cell/yeast/bacteria expression systems. Skilled in monoclonal/bi-specific/tri-specific antibody and protein purification with AKTA purification platform using the methods of affinity chromatography, Ion Exchange Chromatography, hydrophobic interaction chromatography, size exclusion chromatography. Extensive expertise in protein characterization and analysis, including SDS-PAGE, HPLC, CE, cIEF, DSF/SLS, DLS, LC-MS-MS, ELISA, Western Blot, kinetics assay, endotoxin quantitation (& removal), , etc. Comprehensive expertise in molecular biology, including molecular cloning, PCR, RT-PCR, qPCR, etc. Skilled in cell culture, including mammalian CHO & HEK 293 cells, insect cell, yeast cell, E.coli and cancer cells. Excellent leadership on leading a group to perform projects and the communicating with upstream and downstream groups. Highly organized, motivated, team-oriented, work independently and collaboratively
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Senior Director, Protein ScienceAb Studio Oct 2022 - PresentHayward, California, Us -
Director, Protein ScienceAb Studio Oct 2021 - Sep 2022Hayward, California, Us
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Associate Director, Protein ScienceAb Studio Jan 2021 - Sep 2021Hayward, California, Us
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Scientist, Protein ScienceAb Studio Aug 2018 - Dec 2020Hayward, California, UsMonoclonal/bi-specific/Tri-specific antibodies and proteins expression, purification, characterization and formulation:• CHO and HEK293 cell culture and for protein/antibody expression.• Transient transfection and stable cell line development for Monoclonal/bispecific/Tri-specific antibodies and proteins. • Monoclonal/bi-specific/Tri-specific antibodies and proteins purification via affinity chromatography, Ion Exchange Chromatography, hydrophobic interaction chromatography and Size Exclusion Chromatography using AKTA purification platform. • Antibody and protein characterization by SDS-PAGE, HPLC, CE, DSF/SLS, DLS, cIEF, BLI, endotoxin quantitation.• Formulation optimization for the Monoclonal/bi-specific/Tri-specific antibodies.• Antibody and protein functional assay using the methods of ELISA, FACS, kinetics assay, etc.
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Director, Medical BiologyMore Health Inc. Jun 2015 - Jul 2018San Mateo, California, Us• Leading the Medical Biology group to bridge doctors and patients, and provide the newest treatment method/Drugs and MOA info to the patients.• Study pathologies of different kind of diseases, such as lung cancer, leukemia, myeloma, etc. • Study the mechanism of action of the related drugs, including the target drugs (e.g.ceritinib, rucapanib), the immunotherapy drugs (e.g. PD1/PD-L1 antibodies, CD38 antibody), and so on. -
Postdoc ScientistUc Davis Health Mar 2010 - Jun 2015Sacramento, Ca, UsInvestigated the immunity and stress related cell signaling pathways (p38 MAPK, JNK, etc) in Malaria vector mosquito, revealed that inhibition of mosquito p38 or JNK signaling pathways significantly reduced malaria parasite infection, and dissected the detailed mechanism at gene/protein/metabolite levels both in cell and in vivo. • Analyzed mosquito genome sequence and identified 17 MAPKs genes; cloned P38 MAPK and two JNK genes.• Revealed that p38 MAPK take part in the regulation of mosquito anti-malarial peptides through Lucifarase reportor assay in cells and qPCR assay in vivo. • Performed the proteomics experiment (LC-MS/MS) to study the function of mosquito p38 MAPK pathways during infection. The result indicated that p38 MAPK inhibition decreased the antioxidant defense, the mitochondrial protein synthesis, OXPHOS and the protein translation.• Revealed ROS level increased after p38 inhibition with cofocal fluorescence microscopy, and further study indicated that p38 inhibition reduced mosquito’s survival.• Testify the metabolism regulation by p38 inhibition through metabolite analysis on Pyruvate/Lactate level. • JNK function was studied through recombinant expressing two mosquito JNK genes ( with vector pcDNA3.1) in mammalian CHO cell and insect ASE cell; and also using the RNAi (Morpholino) in vivo.• Cooperated with colleagues studying IGF (Insulin-like growth factor) signaling and PKC (Protein kinase C) signaling in malaria vector mosquito. -
Postdoctoral ScholarUniversity Of New Mexico Sep 2008 - Feb 2010Albuquerque, Nm, Us• Recombinant expressed a fibrinogen-related protein (FREP4) from Schistosome vector snail – including the entire protein and the two domains (Immunoglobulin, FBG) in yeast expression system(pPICZα vector and Pitch pastoris strain X-33), and purified the recombinant proteins through affinity chromatography, analyzed their bioactivities on inhibiting bacteria, binding Schistosome SEP, and killing Schistosome in vitro.• To enhance the expression of the entire FREP4 protein, several strategies were applied: 1) try other yeast strains GS115 and KM71H, 2) synthesized a new gene sequence with best synonymous codon bias to Pitch pastoris yeast to let the gene better expressed, 3) transform the yeast with 4X or 8X target genes in one vector, 4) express the protein through different vectors such as the pET32a, pGEX-4T-1, p-ColdI-GST in bacterial expression strain BL21(DE3)plysS, and finally found a way to express enough soluble recombinant FREP4 protein. -
Research AssistantChinese Academy Of Sciences Sep 2005 - Jul 2008Beijing, Cn• Identified and characterized two novel immune-related SPI genes from scallops: gene cloning, sequence analysis, gene expression, and genes regulation after infection.• Expressed recombinant proteins of SPIs in bacterial system, purified the recombinant proteins through affinity chromatography, then proved their inhibitory activities to proteases and get the Ki through kinetics assay. -
Research AssistantOcean University Of China Sep 2002 - Jul 2005青岛市, Cn• Cloned and sequenced the18S, 5.8S, 28S rRNA and ITS domains of the 7 species/strains from the Genus Prorocentrum (Red-tide causing species) in China Seas.• Analyzed all the sequences and developed a set of species-specific DNA probes for an automatic red-tide detection system.
Bo Wang Skills
Bo Wang Education Details
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University Of Chinese Academy Of SciencesMolecular Biology And Biochemistry（Immunology） -
Ocean University Of ChinaChemistry
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Ocean University Of ChinaBiology
Frequently Asked Questions about Bo Wang
What company does Bo Wang work for?
Bo Wang works for Ab Studio
What is Bo Wang's role at the current company?
Bo Wang's current role is Sr Director at Ab Studio Inc. Protein Science.
What is Bo Wang's email address?
Bo Wang's email address is oc****@****ail.com
What schools did Bo Wang attend?
Bo Wang attended University Of Chinese Academy Of Sciences, Ocean University Of China, Ocean University Of China.
What are some of Bo Wang's interests?
Bo Wang has interest in Table Tennis, Badminton, Reading, Hiking, Tennis.
What skills is Bo Wang known for?
Bo Wang has skills like Cell Culture, Molecular Biology, Western Blotting, Pcr, Qpcr, Microscopy, Biochemistry, Cell Signaling, In Vitro, Research, Proteomics, Molecular Cloning.
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