An open source Redis database metrics visualization tool - www.rediscope.com• Designed live metrics visualization with customizable data collection intervals and ability to set a maximum number of visualized data points for enhanced data resolution.• Initiated a PostgreSQL database for relational data storage and accessibility in an ACID compliant structure with strict schemas, enabling secure concurrent connections from multiple clients. • Built functionalities for improved user control. Secure login, store, access, and delete database aliases and URLs. • Prepared for production, application dockerization for quick and reliable deployment on AWS Elastic Beanstalk. Created a custom domain name using AWS Route53. • Developed under tech accelerator OSLabs (opensourcelabs.io).

An open source scientific application for data capture - microbe growth in shake flasks• Engineered a biotech geared application for tracking experimental conditions, key events, and results in a SQL database to minimize data redundancy through proper use of foreign keys. • Use of single page architecture and implementation of React Router for effective page navigation. Improved user experience and reduced network traffic through reduced server calls. • Leveraged React’s Context API to store application data to directly access and update state without manually passing information through the component tree at each level. Reduced code complexity and simplified state management, resulting in intuitive data flow.

• Upstream process development for production of fusion proteins based on AMT’s carrier technology platform. Fermentation process development and production for animal studies for lead therapeutic proteins, early-stage pipeline, and developmental candidates.

• Implemented Thermo Scientific single-use 30L, a direct scale down model used to operationalize and optimize the manufacturing 300L. Fermentation recipe transfer, scale-up from 2L, and validation. • Client-side team lead for Benchling implementation, a cloud-based platform for biotechnology research and development. Established entity schemas and material relationships within an Entity-Relationship model framework using a SQL database. • Led development and testing in a staging environment and completed the migration of configurations (schemas, team structures, notebook templates, etc.) into the production environment.

Fermentation Department - New Technology Team • Implemented Sartorius Ambr250 small-scale, single-use bioreactors (high-throughput, automated liquid handling: cell count, inoculation, sampling, etc) for engineered yeast across multiple fermentation recipes and projects. Fermentation recipe transfer, scale-down, system validation, validation across projects. • Collaborated with software engineers to integrate/automate entity creation and data collection based on key events. Communicated and supported troubleshooting to diagnose missed event captures. • Led well-plate seed development for the Amber250 platform. Worked with software engineers to address UI, material flow, and identify data entry points.

• High-Throughput Screening Lead: Developed and validated methanotroph growth and screening in the 96WP format. Increased microtiter screening capability and throughput by 4X. Automated assays by building liquid handler methods to handle additional throughput. • Improved growth rates and titer across small-scale formats by 200-300%, allowing for improved breakout in performance. Reduced microtiter plate standard deviation and increased dynamic range of testing.

Genencor Dupont - Biochemistry Group Automation and Data Visualization Team • Conduct library screenings: Bacillus subtilis growth, protein (expression, harvest, quantification), performance evaluation in 96-well microtiter plate assays, and hit picking lead molecules for further analysis. Coordinate third-party sequencing, mutation reports, and data file generation. • Developed High-performance liquid chromatography (HPLC) integration methods for amylase quantification, resulting in a significant reduction in data processing time.

Genencor Dupont - Applications Group Grain Applications Research Team • First generation bioethanol production Project: Screen biorefinery components for simultaneous saccharification and fermentation (SSF). Develop industry specific model systems for bioethanol production and performing fermentation evaluations in micro-titer plates, shake flasks, ANKOM Gas Production systems, and DASGIP bioreactors. • Responsible for applications test design, execution, and analysis of experiments for first generation bioethanol simultaneous saccharification and fermentation (SSF) biorefinery components: alpha-amylase, gluco-amylase, auxiliary enzymes, yeast and fungi.• Perform performance validation and develops small-scale model systems to perform scale-up testing, before and throughout development. Contributes to project strategies, reports, summaries, protocols, and quantitative analyses, and presents results of data to team.• Completed 100g SSF shake flask fermentation scale-up to 1 liter DASGIP bioreactor. Modeling applications to mimic anaerobic conditions observed in industry enabled determination of industry relevant breakout in fermentation performance.• Standard Operating Procedure (SOP) creation - DASGIP Bioreactor System for first generation fuel ethanol production.

Genencor Dupont - Applications Group Grain Applications Research Team • Responsible for applications test design, run, and analysis for first generation bioethanol simultaneous saccharification and fermentation (SSF) biorefinery components: alpha-amylase, gluco-amylase, auxiliary enzymes, yeast and fungi.• Experience in screening, candidate selection, optimization of production parameters, and product stability assessment.• Standard Operating Procedure (SOP) creation - ANKOM system. Assay Optimization - Residual Starch quantification. Mentorship - Student intern training, management, and supervision.• Applications research contributor to grain ethanol enzymes and functional bio products Dupont’s Total Performance System (TPS) commercialization in 2013• Attended 2014 Alcohol School Montreal - fuel ethanol and distilled beverage unit processes• Techniques: FLOWCAM particle analysis instrument for microorganism quantification, HPLC, cloning, culturing, SDS-PAGE, PCR, gel electrophoresis, distillation, spectrophotometry, chromatography, assay development, fermentation.• Data visualization in Spotfire, MindManager, and Microsoft Office (Word, Excel, PowerPoint). Experience with computer based chemical and biomolecular process modeling/analysis utilizing Unisim, Matlab, Femlab, Design Expert, and Polymath.