Bo Wang Email & Phone Number

Cambridge, Ma, Us

Cambridge, Ma, Us

Columbia, Maryland, Us

Waltham, Massachusetts, Us

上海, Cn

Shanghai, Shanghai, Cn

Lexington, Massachusetts, Us

Boston, Ma, Us

Provided various cell lines (including chick embryo atrial myocytes, mouse primary atrial and ventricle myocytes) to several projects.Discovered impaired parasympathetic response in type I diabetic mice by measuring L-type calcium channel current (using patch clamp technique) and single cardiomyocytes contractility and calcium transients (using Ionoptix imaging system)Trained scientists and technicians in cardiomyoctye isolation and cell culture.Developed abdominal aortic aneurysm mouse model to study potential therapeutic targets.

Boston, Ma, Us

Animal skills In vivo: Animal surgery including aortic constriction and coronary artery ligation .Made abdominal aortic aneurysm mouse model and studied potential therapeutic target. Performing PK and efficacy studies in Hemophilic mice that involve dose administration by tail vein. Intraperitoneal ,subcutaneous or intradermal injections. Blood collection via vena cava. Hemostasis challenge by tail clip or tail vein dissection.In vitro: Cell/Tissues cultures.Primary cell culture including adult and neonatal cardiomyocytes, atrial cells, adult cardiac progenitor cells, bone marrow derived stem cells form rodents (mouse and rat) and large animals (sheep and pig), DNA, RNA isolation and purification from cells and tissue, Embedding tissues and paraffin section, Immunohisochemistry , Hematoxylin and Eosin stainingWestern blots, PCR, RT-PCR , Protein and DNA gel electrophoresisEndothelial cell isolation and migrationAdhesion assays with monocytes and endothelial cell monolayersTransformation of chemically competent bacteriaTransfected mammalian cells with siRNA Mouse colony maintenance, including tagging, breeding, and genotyping. Organized and Laboratory material ordering and keep up.Trained scientists and technicians in cardiomyoctye isolation and cell culture.Coordinate projects between researchers internally and/or externally.Transformation of chemically competent bacteriaCultured various human and mouse endothelial cell lines and primary cellsTransfected plasmid DNA expression vectors into mammalian cells in culturePerformed adhesion assays with monocytes and endothelial cell monolayersTransfected mammalian cells with siRNA Performed endothelial cell isolation and migration assays Performed Elisa assayEmbedding tissues and paraffin sectionImmunohisochemistry , Hematoxylin and Eosin stainingAnimal surgery including aortic constriction and coronary artery ligation

Us

Primary cell culture including adult and neonatal cardiomyocytes, atrium cells, adult cardiac progenitor cells, bone marrow derived stem cells form rodents (mouse and rat) and large animals (sheep and pig)Mouse colony maintenance, including tagging, breeding, and genotyping Animal injections (mouse and rat) including subcutaneous, intraperitioneal, intravenous, intramuscular, intrademal.DNA, RNA isolation and purification from cells and tissueRT-PCRTail-tip collection and genotyping Protein isolation from cells and tissueProtein and DNA gel electrophorsisWesternblot analysisImmunohistochemistryLearned LSM 710/LSM 710NLO and Confocor3 microscopy and pictures takenResponsible for training students and postdocsCoordinate projects between researchers internally and/or externally

Boston, Ma, Us

Some experience in animal surgery including aortic constriction and coronary artery ligation Chemicals and reagents ordering and lab general up keep. Protein isolation and Westernblot analysisLangendorff perfusion of isolated murine heartsCulture room upkeepAnesthesia techniques in rodentsProject coordination with other researchers/investigators

Residency

M.D, Medicine

Biomedical Science

B.D. (M.D.), Medicine