Braden Keiser, M.Sc Email & Phone Number

Tewksbury, Massachusetts, United States

As a freelance bioinformatics analyst, I specialize in troubleshooting DNA, RNA, and protein mutagenesis strategies, encompassing everything from sequence analysis to structural interpretation. My services include:• Omics Data Analysis: Parsing sequence information for quality control, trimming, and expression profiling;• Automated Scripting: Developing streamlined protocols for Linux systems, compatible with both local and high-performance computing (HPC) environments.• Literature Review: Conducting thorough reviews to support data interpretation and research.Proficient in Python and R, I prioritize creating backward-compatible code to support dynamic workflows and enhance research capabilities without disrupting existing frameworks. This role builds on my collaborative research experience at Shanghai Jiao Tong University, where I partnered with the Thailand National Institute for Genetic Engineering on biorefinery initiatives.With a year of hands-on cell biology lab experience, I am eager to return to benchwork and am passionate about developing experimental sequencing strategies and constructing omics libraries. From the command line to the cell line, I am dedicated to deriving actionable insights from complex biological systems.

Shanghai, China

• Computational (80%), experimental (20%) researcher in collaboration between bioinformatics lab at SJTU and Thailand's National Institute for Genetic Engineering;• MSA of protein queries and construction of homology model (R, Bioconductor, NCBI)• Verified plastic-degrading enzyme production by proteomic and WGS experimental analysis protocols;• MD Simulation (GROMACS, Amber) for assessing mutant thermostability and catalytic profiles;• QM Analysis (Gaussian, Orca) of key chemical systems, e.g. the examination of pi-amide interactions; • In-house preparation of computational analyses of trajectory and QM data (R and Python); • Scripting (Bash, Tara and SLURM HPC server submission-compliant) of protocols for conducting MD simulation and QM analysis for use by other researchers;• Quarterly reports and presentations to researchers, business leaders, and assistants;• Leader of communication between the work of our Chinese lab and our collaborators in Thailand;• Conducted peptide separation using nano-LC-MS/MS and identified proteins with Mascot

Shanghai, China

• Proficient in cell culture, including initiation, subculturing, media prep, and cell counting• Recombinant DNA cloning with Plasmid Design, Western Blot, and Sanger Sequencing• Utilized PCR, qPCR, and Sequence Library Construction;• Explored z-stacking fluorescent microscopy with image analysis and segmentation via Random Forest Classification. • Developed proficiency in Western Blot, DNA/RNA isolation, extraction, and amplification. • Installation and dependency maintenance of Unix PBS system for lab submission server

Kunming, Yunnan, China

Following my undergraduate studies, I moved to the capital city of Kunming in Yunnan province, China to teach English. With my science background, I was able to teach English through an interactive science program to elementary and middle school students. This was a wonderful time of my life where I learned much about my own cultural preconceptions, the inspiring diversity of ethnic cultures in rural China, and conveying the importance of science education across a wide swathe of youth backgrounds.

• Extracted blood samples from Triatominae and identified presence of T. Cruzi• PCR, gel electrophoresis, and data entry for mapping disease spread in Central America

Master Of Science - Ms, Biology: Bioinformatics And Computational Biology

Thesis on: Thermostabilizing and Catalytically-enhancing Effects of Novel Green Enzymes for Bio-Plastic Degradation and Waste.

Bachelor Of Arts - Ba

Undergraduate Semester