Lake County Site Pre-analytical Coordinator:Routine preparation of standard calibration curves using Tecan D300e liquid handling dispensers Daily sample preparation of various biologics including but not limited to: whole blood, plasma, serum, feces, urine, bile, and various tissues or organsDaily extractions using methods such as protein precipitation, liquid liquid extraction, salt assisted liquid liquid extraction, solid phase extraction, and custom designed assay requests prepared for small molecule LCMS analysis and interpretation Variable automation experience: Hamilton liquid handlers (starlet, star, starplus), Omni homogenizers, Scinomix automated tube labeling, and Sirus custom designed automated platforms Validation of novel automation processes and methodsExtensive research LIMS experience in sample preparation regarding daily lab maintenance such as sample/compound storage, batch, and run list creation Management: direct report, summer workers, summer interns, and extensive onboarding experience External and internal vendor communication and coordination SOP writing, creation and updating existing protocolsCell disposition:Cell titer glow assays: immunofluorescence imaging Cell culturing: suspension and adherent cells, splitting

Chemist I working with the pre-analytical and DMPK teams.

- Preform basic and complex specimen analysis within urinalysis, hematology/coagulation, and blood gas departments. - Daily quality control and corresponding documentation for automated instruments. - Organization, preparation, and packaging of laboratory specimens to be delivered to reference laboratories.

- Independent management of wild type and knockout mouse colony.- Provided training and guidance for students unfamiliar with laboratory practice. - Responsible for design and production of written RNA extraction experiment protocol. - Organized daily documentation in laboratory journal. - Confocal imaging and fluorescent microscopy training. - Polymerase Chain Reaction (PCR) and Gel Electrophoresis.- RNA extraction from rodent cortical astrocytes and organs.- Serological immunofluorescence experiments: rodent cortical astrocytes and brain tissue. - Post-natal mouse cortical astrocyte dissection and cell culture maintenance.- Solution preparation and management.- Efficient with sterile laboratory techniques.

- Lead and trained newly hired rehabilitation aides. - Assisted the office coordinator with patient intake screening and scheduling, daily paperwork, and insurance preauthorization. - Independently lead patients through personalized therapeutic exercise routines.- Developed therapeutic exercise routines for patients when requested.

- Prepared food from fryer, grill, and salad stations in a fast paced environment. - Developed unique meals for weekly specials.

- Catered weddings, private events, and concerts in a large lounge venue. - Trained and managed new employees. - Responsible for ordering food and kitchen supplies. - Lead catering and layout design for weddings/concerts.- Food safety training.