Led and executed multiple studies focused on the role of gamma delta (γδ) T cells in persistent HIV-1 infection. Unconventional lymphocytes such as γδ T cells are critical mediators of tissue surveillance, but their involvement in anti-viral immunity is poorly understood. In the main body of my dissertation, I uncovered a juxtaposing role for circulating and tissue-resident γδ T cells in persistent HIV-1 infection. Through high multiparameter flow cytometry, gene expression analysis, and functional assays I characterized a subset of highly differentiated, cytotoxic γδ T cells that possess potent anti-HIV capabilities in virally suppressed people with HIV. Conversely, we found a separate, distinct subset of CD4+ γδ T cells within the gut. Utilizing ultra-sensitive methods in nucleic acid and protein detection, we demonstrated that this tissue-resident subset contain HIV-1 DNA and act as a latent reservoir for the virus. Collectively, our data unifies conflicting hypotheses from previous studies by describing how γδ T cells can simultaneously be involved in anti-HIV immunity and also contribute to infection. Building off of the findings from the previous study, we explored the potential use of allogeneic γδ T cells for HIV-1 cure. Similar to other unconventional lymphocytes, γδ T cells recognize target cells through MHC-independent mechanisms. Using in vitro assays for cytotoxicity, degranulation, and cytokine secretion, I demonstrated the specific ability of allogeneic γδ T cells to eliminate HIV-infected CD4+ T cells and Macrophages.

Conducted translational research as a part of the U.S. Military HIV Research Program's (MHRP) Viral Sequencing Core. Tasked with the design, implementation, and troubleshooting strategies to generate infectious molecular clones of circulating HIV variants isolated from global clinical trials (Sub-Saharan Africa, Southeast Asia, and US). Utilized HIV clones to assess neutralizing antibody responses from vaccinated individuals and investigate critical aspects of viral transmission including the establishment of transmitted/founder viruses within primary peripheral blood and tissue samples.

Carried out high-throughput screening of protein-protein interactions using the Yeast Two-Hybrid System to enhance hypothesis testing on host-pathogen interactions between agricultural crops (e.g. soybeans) and the Phytophthora genus. Independently managed large-scale experiments, maintained bacterial and yeast cultures, and presented analyses at weekly lab meetings.