• Cultured, then harvested Escherichia coli to meticulously purify ample stocks of plasmid DNA for chromatography purposes• Transformed chemically competent Escherichia DH5a cells with various plasmids and carried out ddPCR• Examined pDNA quantity and quality using picogreen assays, qubit assays, and gel electrophoresis techniques• Tested dynamic binding capacity and flow distribution of various membranes using CEX and AEX chromatography processes• Detected HEK HCPs and benzonase impurities within samples using enzyme link immunosorbent assays (ELISAs)• Employed & modified TFF protocols to remove beznonase, DNA, and human embryonic host cell proteins from the retentate

• Designed a method to maximize pDNA recovery and maintain its quality, with a focus on efficiency and preservation • Demonstrated an alternative technique uniquely tailored for the comprehensive and broad quantification of our therapeutic• Successfully separated 60% of a novel isoform of DNA from a variety of impurities with tangential flow filtration• Identified a straightforward method to eliminate dissolved gas from the homogenate, streamlining the process effectively• Evaluated the activity of proteases and nucleases, demonstrating self-autonomy in experimental planning and execution• Northeastern accredited co-op position

• Conducted experiments to assess chemical reactions under diverse conditions, illustrating competence in experimental design• Explored feasible manufacturing alternatives to extend chemical reaction durations for AI to assess reactions more accurately• Resolved which gastrointestinal microbials to selectively test and present to users on a phone application