Brett Schroyer Email & Phone Number

Burlington, MA, US

• Cultured, then harvested Escherichia coli to meticulously purify ample stocks of plasmid DNA for chromatography purposes
• Transformed chemically competent Escherichia DH5a cells with various plasmids and carried out ddPCR
• Examined pDNA quantity and quality using picogreen assays, qubit assays, and gel electrophoresis techniques
• Tested dynamic binding capacity and flow distribution of various membranes using CEX and AEX chromatography processes
• Detected HEK HCPs and benzonase impurities within samples using enzyme link immunosorbent assays (ELISAs)
• Employed & modified TFF protocols to remove beznonase, DNA, and human embryonic host cell proteins from the retentate

Paris, France, FR

• Designed a method to maximize pDNA recovery and maintain its quality, with a focus on efficiency and preservation
• Demonstrated an alternative technique uniquely tailored for the comprehensive and broad quantification of our therapeutic
• Successfully separated 60% of a novel isoform of DNA from a variety of impurities with tangential flow filtration
• Identified a straightforward method to eliminate dissolved gas from the homogenate, streamlining the process effectively
• Evaluated the activity of proteases and nucleases, demonstrating self-autonomy in experimental planning and execution
• Northeastern accredited co-op position

New York, NY, US

• Conducted experiments to assess chemical reactions under diverse conditions, illustrating competence in experimental design
• Explored feasible manufacturing alternatives to extend chemical reaction durations for AI to assess reactions more accurately
• Resolved which gastrointestinal microbials to selectively test and present to users on a phone application

Master'S Degree, Biotechnology

Bachelor'S Of Science