Brian Coley Email & Phone Number

Raleigh-Durham, North Carolina Area

Mcleansville, North Carolina

• Use the information presented in due diligence reports, covering all project functional areas, to define the scope for an acquisition project
• Develop the project charter and project plan based on the defined scope and key requirements
• Collaborate with key stakeholders and resources to identify and manage any risks that may arise during the course of the project
• Organize and schedule project team meetings
• Facilitate project team meetings and apply updates to project task items as needed
• Immediately address any issues that arise and escalate to the correct authority via proper the channel

Burlington, NC

• Performed enzyme-based nucleic acid amplification for the detection of Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas. The assay utilized a fluorescent probe for end point detection
• Worked together in a team environment to implement clinical testing of patient approximately 12,000 patient samples daily
• Communicated with management and team members to proactively address any issues that could possibly affect lab throughput and efficiency

Greensboro, NC

• Researched the development of an ELISA-based assay for the capture of free Argonaute (AGO) 2 protein in complex with miRNA Let-7a, followed by extraction and detection of miRNA Let-7a via qPCR

Greensboro, NC

• Provided instruction and support for the General Chemistry lab course as a Teaching Assistant
• Provided weekly tutoring and support for General Chemistry students on course subject matter
• Maintained a safe laboratory environment and ensured that students adhered to policies regarding laboratory safety

Burlington, NC

• Ensured effective quality control within the laboratory by upholding GCLP, CLIA, and CAP standards and enforcing adherence to SOP protocols by all technologists
• Coordinated a daily plan of action for the 10 technologists I oversaw
• Ensured that expected testing time-frames were met, and relayed any pending delays in testing to departmental customer service
• Collaborated with management to re-evaluate current workflow trends and determine areas in which the department could be more efficient
• Addressed scheduling needs due to staffing and re-assigned technologists accordingly to circumvent any possible lapse in patient testing
• Reviewed all work performed by technologists prior to the final results being reported to clients

Burlington, NC

• Performed validation of a protocol for testing ‘low-volume’ PAP samples using the Qiagen Hybrid Capture Assay kit
• Performed validation of the Protodyne Radius automated specimen preparation system for Human Papilloma Virus (HPV)
• DNA extraction from patient PAP samples to be tested for HPV
• Detection of HPV from patient PAP samples using the Qiagen Hybrid Capture HPV assay kit
• Detection of HPV from patient PAP samples Hologic ASR assay kit
• Detection of Methicillin-Resistant Staphylococcus Aureus (MRSA) virus from patients samples using real-time PCR

Greensboro, NC

• Performed cell culture of E. Coli cells
• Transfected E. Coli cells with X-gal plasmid vector containing a Wnt-5a gene sequence
• Harvested E. Coli cells that exhibited successful uptake of the plasmid vector and used these cells to prepare new cell lines containing the target plasmid vector
• Performed plasmid DNA extraction from E. Coli cells
• Performed agarose gel electrophoresis of cellular DNA extracts
• Extracted and purified plasmid DNA from agarose gel using resin-based methods

Master Of Science (M.S.), Biochemistry

B.A., Biology

Activities and Societies: Kappa Alpha Psi Fraternity, Inc., Neo-Black Society Choir, Trombone Choir, University Band