Brian Holmberg Email and Phone Number
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Brian Holmberg is a Principal Scientist at Novartis Institutes for BioMedical Research (NIBR) at Novartis Institutes for BioMedical Research (NIBR). He possess expertise in life sciences, pharmaceutical industry, biotechnology, molecular biology, biochemistry and 11 more skills.
Novartis Institutes For Biomedical Research (Nibr)
View- Website:
- novartis.com
- Employees:
- 5
- Company phone:
- +41 61 324 11 11
- Company email:
- eric.althoff@novartis.com
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Principal ScientistNovartis Institutes For Biomedical Research (Nibr) Jan 2008 - PresentBasel, Baselstadt, ChAt Novartis I have worked in antibody discovery, with particular focus on yeast and phage display, and high throughput automated assay design and programming,Using yeast display, I improved the potency of an anti-BCMA antibody over 1000x, leading to an IND filing for a CD3 bispecific antibody and a granted patent. I designed and created the maturation library, performed the selection campaign, subcloned and expressed the bispecific antibodies in microplate scale, and ran high throughput activation assays to select the lead molecules.For that project, I developed, optimized, and programmed a variety of automated processes which would later become standard workflows in our group, including a plate based miniprep protocol, 96 well mammalian cell transfection and expression, and a high throughput activation assay. Another useful assay I designed and programmed is a high throughput flow cytometry binding assay, with labeling on a Biomek i7 system and readout using an iQue analyzer. I used this protocol to identify single domain antibody hits for a radioligand therapeutic project. This has become very commonly used in our group as a primary screening method.
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Technical AssociateUniversity Of Rochester Mar 2003 - Nov 2007Rochester, Ny, UsAt University of Rochester I worked in the department of Biochemistry and Biophysics and investigated ion transporter activity and structure using a variety of radioactive and fluorescence assays. I measured fluorescent inhibitor binding in saccharomyces - expressed transporters using flow cytometry. I purified membrane proteins and domains and measured conformation change using luminescence resonance energy transfer. As part of a multidisciplinary collaboration, I helped develop surface coatings for immobilization of membrane proteins and measurement using single pair fluorescence resonance energy transfer.
Brian Holmberg Skills
Brian Holmberg Education Details
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Binghamton UniversityBiochemistry
Frequently Asked Questions about Brian Holmberg
What company does Brian Holmberg work for?
Brian Holmberg works for Novartis Institutes For Biomedical Research (Nibr)
What is Brian Holmberg's role at the current company?
Brian Holmberg's current role is Principal Scientist at Novartis Institutes for BioMedical Research (NIBR).
What is Brian Holmberg's email address?
Brian Holmberg's email address is br****@****tis.com
What is Brian Holmberg's direct phone number?
Brian Holmberg's direct phone number is +158527*****
What schools did Brian Holmberg attend?
Brian Holmberg attended Binghamton University.
What skills is Brian Holmberg known for?
Brian Holmberg has skills like Life Sciences, Pharmaceutical Industry, Biotechnology, Molecular Biology, Biochemistry, Drug Discovery, Cell Culture, Hplc, Microsoft Office, Phage Display, Protein Purification, Yeast Display.
Who are Brian Holmberg's colleagues?
Brian Holmberg's colleagues are Kim Chambers, Christian Maurer, Saskia Lellé, Olga Vorobeva, Doga Ozgul (Cevrim), Heidi Mergentaler, John J Fritsch.
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