Brooke Tomlinson Email & Phone Number

Waltham, Ma, Us

Leading a team of incredibly talented FAS supporting our Sequencing product applications at Thermo Fisher Scientific

Waltham, Ma, Us

Tampa, Florida, Us

• Developed and presented lectures and designed interactive group exercises on RNA-sequencing technologies, experimental design, and data analysis pipelines.• Demonstrated end point data analysis, handling, and visualization using diverse tools: R, Python, CLC Workbench, Perl, Circos, Microsoft Excel.

Tampa, Florida, Us

• Research experience investigating molecular mechanisms of virulence for BSLII-level pathogens in the search of novel therapeutic targets; resulted in 11 publications and dissertation award.Publications: https://orcid.org/0000-0001-6561-7931 • Acted as point of contact for next-generation sequencing technologies including whole genome sequencing, RNA-Seq, and ChIP-Seq. Provided technical and scientific assistance for eleven internal scientists and ten external collaborators, including experimental design and protocol optimization tailored to project needs. Utilized Illumina, Ion Torrent, Agilent, Roche, and Sanger platforms.• Trained, presented, and demonstrated next generation sequencing, RNA isolation, Bioanalyzer/TapeStation/Qubit nucleic acid assays, PCR, qPCR, proteomics, cellular imaging, zymography, northern blot, 5’/3’ RACE, electromobility shift assays, murine infection models, and numerous molecular cloning techniques.

Tampa, Florida, Us

• Spearheaded conversion of wet lab microbiology courses to online format and coordinated nine teaching assistants to ensure a smooth transition amidst the Sars-CoV2 pandemic; resulted in award for teaching excellence.• Instructed laboratory for General Microbiology and Biological Processes I courses of 40-48 students and trained five novice teaching assistants to maintain instructional continuity.• Demonstrated laboratory equipment use including compound microscope, micropipettes, gel-electrophoresis, autoclave, and thermocycler. • Trained students to perform aseptic technique, cell content imaging and analysis, cell viability assays, and isolation, characterization, and identification of pathogenic organisms.• Managed the preparation of laboratory supplies for 20+ laboratory teaching sections of 20-24 students each, including differential and selective media, consumables, and bacterial cultures.

• Performed cell-based assays for research and product development of proprietary insect cell lines, including cell viability assays, transfection, clone selection, proliferation assays, viral plaque assays, and cell content analysis via fluorescence microscopy.• Designed experiments to test efficacy and consistency of products during development while establishing quality control standards.• Established multi-disciplinary approaches for invasive species remediation to expand biopesticide product portfolio; assisted with foundation of daughter company, Lepidext, to lead development of these niche products.• Maintained an accurate written record of all work in a permanent laboratory notebook, edited technical documentation, and refined SOPs.

• Assisted with the routine care of hospitalized animals including cats, dogs, and rabbits. As the resident assistant, I also provided general oversight of the clinic and after-hour recovery care for critical patients.

Lexington, Ky, Us

• Part time laboratory technician in collaboration with Paratechs Corporation.• Performed viral DNA extraction, PCR, infectious agent administering, phenotypic evaluation by dissection, and insect colony rearing. This interim position served as an introduction to entomology research prior to employment at Paratechs Corporation.

• Performed DNA extraction from tissue and blood samples, PCR, gel electrophoresis, microscopy, and karyotyping.Developed independent research projects for the University of Kentucky Department of Veterinary Science. • Investigated the genetic basis of equine susceptibility to Equine Arteritis Virus (EAV) infection by screening perissodactyl family for the CXCL16 gene (associated with genotypic susceptibility to EAV), leading to completion of independent research project.

Sydney, 2010, Au

I worked for Contact Centres Australia in their outbound call center on behalf of the Australian Red Cross. I was responsible for educating the public about available relief programs such as Telecross, the Breakfast Club, and Disaster Preparedness while seeking donations to support the aforementioned programs.

After completing a training overview of the veterinary practice, I filled a short term position as a temporary veterinary assistant and receptionist. Responsibilities included routine care for the small animal patients, scheduling appointments, communicating with clients, and managing sales.

Doctor Of Philosophy - Phd, Cell/Cellular And Molecular Biology

Bachelor’S Degree, Agricultural Biotechnology, Pre-Veterinary Studies

Study Abroad, Animal Sciences