Resourceful research-oriented cell biologist with extensive experience in assay development, particularly fluorescence techniques utilizing imaging microscopy (high-content, epi-fluorescence, confocal, deconvolution), flow cytometry, fluorometry, fluorescence spectroscopy, and microplate readers related to bacteria, yeast, and mammalian cell usage. A highly motivated individual with many years of hands-on tissue culture experience: primary cell isolation and propagation, protein overexpression and knockdown utilizing transfections and retroviral transduction (stable and transient), stable clone isolation/characterization. Protein overexpression/knockdown was assessed using Western blotting, ELISA, High Content Imaging, flow cytometry, and microplate readers. A proven track record in development of new reagents, techniques, and instrumentation for a wide range of biological research applications. Next generation sequencing DNA/RNA bulk, single cell and single nuclei with epigenetic characterization V(D)J , TCR/BCR and CITESeq. Specialties: High content imaging and quantitative analysisFlow cytometryCellular assay development (proliferation, cytotoxicity, apoptosis, and reporter gene assays)Tissue culture: primary cell isolation, characterization, and propagation (creation, passage, cataloging, long-term cryopreservation, expansion to large flask/roller bottles, and dose preparation for xenograft/allograft modeling)