Bruce Zamost Email & Phone Number

Cambridge, Ma, Us

Process development for live Dengue VaccineUpstream and Down Stream Process development for a Zika VaccineProduction of Norovirus vaccine antigens in bioreactors using the BVES and insect cell culture to produce virus like particles. Small scale model qualifications and process characterization of upstream processes including cell culture and culture clarification.

Upstream BioSolutions LLC. Seattle, WA (2010– 2014)Principal ConsultantUpstream BioSolutions provides consulting services related to the development and scale-up of upstream bio-processes for the production of microbial derived products. These products can include bio-pharmaceuticals, secondary metabolites, vaccines, biological insecticides, or specialty enzymes. Highly knowledgeable in strain construction and cell physiology of the yeasts Saccharomyces cerevisiae Pichia pastoris and Pichia methanolica. Experienced in fermentation optimization and scale-up of engineered strains of yeasts, fungi and E. coli. Upstream BioSolutions services range from technical support to development in the following areas:• Microbial fermentation optimization and process development for a range of genetically modified organisms including E. coli, Pichia pastoris, Pichia methanolica and Saccharomyces cerevisiae, • Production of bio-active products from Bacillus species and fungi such as Trichoderma reesei and Aspergillus species,• Production of secondary metabolites from Streptomyces, filamentous bacteria and fungi,• Strain selection and media optimization for microbial processes,• Cell harvest and clarification by continuous centrifugation and filtration,• Recovery and refolding of inclusion bodies from E. coli, • Scale – up, scale down models and technology transfer for all upstream processes.Please contact me for assistance with your upstream processing problems.www.upstream-bio.com

• Fermentation optimization for the production of monoclonal antibodies produced by Pichia pastoris.• Developed scale down and scale-up models for the production of monoclonal antibodies by Pichia pastoris.

Seattle, Washington, Us

- Managed a bioreactor and recovery group involved in process development, scale-up and technology transfer for the production of new therapeutic proteins using a variety of expression systems such as CHO, Pichia, and E. coli.- Developed and optimized fermentation processes for the production of biopharmaceuticals using E. coli Developed and optimized cell culture processes for the production of biopharmaceuticals using CHO

Managed a microbial fermentation and recovery group involved in process development for the production of new therapeutic proteins using a variety of expression systems- Developed and optimized fermentation processes for the production of biopharmaceuticals using the Pichia methanolica expression system -Developed methods for screening yeast strains leading to a new Saccharomyces cerevisiae production strain for recombinant human Factor XIII.

• Produced large amounts of secondary natural products in shake flask culture using a variety of organisms including Penicillium , Streptoverticillium, and Streptomyces sp. • Developed and utilized microbial biotransformation methods to modify lead activity.

NOVO NORDISK ENTOTECH INC. Davis, CA (1990- 1994)Fermentation Group, Product Development, Staff Researcher• Developed new fermentations methods for the production of novel, microbially produced insecticidal metabolites.• Developed new fermentation methods for high potency Bacillus thuringiensis formulations. NOVO NORDISK BIOINDUSTRIALS, Danbury, CT (1989 - 1990)Fermentation Microbiology, New Products Research and Development, Staff Researcher Developed new fermentation proceses for industrial enzymes

Microbiology Department, New Products Research and DevelopmentStrain screening, mutant screening, shake flask design, and fermentation design.

Msc, Biological Sciences

Ba, Microbiology