Bryan Smith Email & Phone Number

Dallas, TX, US

Immunoassays Development: Codeveloped immunoassays on platforms like Quanterix Simoa SR-X, PerkinElmer EnVision 2105, and Promega Glomax Explorer.LC-MS/MS assay development: Worked with Charles River Laboratories to develop a highly sensitive method for detection of Tofacitinib in human serum, colonic tissue and fecal samples.BioJet Capsule: Played a key.

Dallas/Fort Worth Area

• Transformed both labs from Immediate Jeopardy (IJ) status to CLIA Compliant.
• Designed, planned and performed LDT validation of 3 immunoassays for specimen validity testing and 10 immunoassays for drug toxicology screening on Beckman Coulter AU680, AU640, and AU400 platforms.
• LC/MS/MS LDT method development, validation design and data analysis for a 70 drug panel on the Waters Xevo TQD platform.
• Sample shipping conditions validation and development.
• Development of data extraction tools for processing and reformatting instrument data into EP evaluator templates for rapid importation of large amounts of data into EP Evaluator.
• Generation of EP evaluator reports for validation

Dallas/Fort Worth Area

• Transformed both labs from Immediate Jeopardy (IJ) status to CLIA Compliant.
• Designed, planned and performed LDT validation of 3 immunoassays for specimen validity testing and 10 immunoassays for drug toxicology screening on Beckman Coulter AU680, AU640, and AU400 platforms.
• LC/MS/MS LDT method development, validation design and data analysis for a 70 drug panel on the Waters Xevo TQD platform.
• Sample shipping conditions validation and development.
• Development of data extraction tools for processing and reformatting instrument data into EP evaluator templates for rapid importation of large amounts of data into EP Evaluator.
• Generation of EP evaluator reports for validation

• Reduced average data analysis time from 3 hours to 30 minutes through development and validation of Ascent LCMS data analysis methods.
• Data innovations server installation, setup, management, and blood chemistry auto verification rules writing/validation.
• LIS validation/implementation/management at 13 labs for Blood Chemistry, Hematology and Toxicology.
• Development of auto flagging system for prescription/LCMS result concordance/discordance for 56 analytes.
• Instrument interfacing for: Roche Cobas 6000, Sysmex XN, Beckman Coulter AU400 and Au640, Agilent 6460 and 6420, Cell-Dyn Ruby, and the Roche Urysis 1100.
• Implementation and management of Bio-Rad Unity Real Time and Bio-Rad Unity connect QC software for tracking blood chemistry and LC/MS/MS QCs.

• Preclinical and Phase 1 Clinical Trial work. Was responsible for aiding in the development of IRB reports and communications.
• Developed an immunoassay (immunofluorescent staining) for 3D modeling and quantitation of biofilms via confocal laser scanning microscopy as well as simultaneous visualization and counting of live/dead Burkholderia.
• Contributed figures to and developed anti-biofilm PPMOs for patent US9790495B2.
• Created a crystal violet biofilm staining assay for rapid screening of newly developed PPMOs using a BIO-TEK Synergy HT 96 well plate reader.
• Minimal Inhibitory Concentration (MIC) studies, Growth curve studies.
• Polymorphonuclear Neutrophil (PMN) studies with PMNs from patients with cystic fibrosis (purification, apoptosis/necrosis flow cytometry studies, phagocytosis and killing with biofilm producers with the aid of PPMOs).

Odessa/Midland, Texas Area

• High Pressure Liquid Chromatography / Tandem Mass Spectrophotometer (HPLC-MS/MS or LC/MS/MS) Operator and Chromatography First Reviewer. Ameritox Ltd. offered urine analysis for the purpose of pain medication.
• Reviewed samples that were being tested for benzodiazepines, opiates, fentanyl, and gabapentins.
• Made calibration curves for every drug being tested.
• One of two individuals that were trusted to make the mobile phase solutions for all HPLCs.
• Development of automated worklist creation for LC/MS/MS batches.
• Programs used for chromatography analysis and mass spec monitoring included Thermo Scientific’s Aria, LC Quan and Xcaliber.

Odessa, Texas

• The purpose of this project was to scale up human hemoglobin production from BL21(DE3)/pHb0.0TM1hug cultures grown in test tubes to large scale (multiple liters)human hemoglobin production with cultures grown in a.
• Managed and trained 4 lab members to ensure the efficient operation of a Bioflo 110 bioreactor.
• Publications from my master’s thesis work: o Smith BJ, Gutierrez P, Guerrero E, Brewer CJ, Henderson DP. Development of a method to produce hemoglobin in a bioreactor culture of Escherichia coli BL21(DE3) transformed.
• Master’s thesis and project.
• Techniques used during project:o Bioreactor operation and Maintenance.o Analyzed approximately 200,000 data points per run per week.o Batch/Constant-rate Feeding, Stepwise Glucose Feeding and Mass Flow Equation Based.

• Taught Human anatomy and Freshman Biology 1 and 2 with up to 24 students per lab.
• Prepared lab lectures and tutoring sessions.
• Graded tests, quizzes, homework and formal lab write-ups.

Okasaki, Japan

• Communicated in English in a way that comprehension could be conveyed to native Japanese speaking students without the aid of Japanese.
• Students ranging from pre-K to high school age.

Midland, Texas

• Prometric Testing Center Manager and Test Administrator.
• Scheduled staff and determined testing schedule.
• Administered exams and ensured that all client testing regulations were followed for over 300 different clients.
• Monitored logs to ensure that all regulations were followed by the staff.
• Held Regular meetings to ensure that all staff members were aware of any new clients or changes to existing client’s regulations.

Midland, Texas

• Managed a Windows NT small business server and 12 associated computers for analysis of student test results and record keeping
• Tutored students in the following subjects: English, Algebra, Trigonometry, Calculus, Chemistry and Biology

Odessa, TX

• Streptonigrin anti-colon cancer drug research.
• Used GC/MS to quantify if changes made to the synthesis protocol for the pyridine ring-C of Streptonigrin resulted in improved yields above the 28% yield achieved from Diels-Alder reactions.

Molecular Microbiology

PH.D. Candidate in Molecular Microbiology

Japanese Language

Master Of Science - Ms, Molecular Microbiology

Activities and Societies: • Awarded the UTPB Biology Graduate Scholarship from January 2009 - May 2011. • Awarded a graduate.

Bachelor Of Science (B.S.), Biology

Graduated with a B.S. in Biology with a minor in Chemistry summa cum laude. • President’s Honor Roll, August 2003 to May 2007. • Recipient.

High School Diploma

• Midland High School Salutatorian • Member of the UIL Science, Math, and Computer Science teams (3 years) • Duke University Talent Search.