Bryan Barney Email & Phone Number

Sacramento, California, United States

Menlo Park, Ca

I use NextGen sequencing and bioinformatics pipelines to discover genetic markers distinguishing 3 easily mislabeled groundfish species. I have developed qPCR assays to determine genotypes at these markers to identify the species of unknown fish samples

Davis

I use sequencing technologies at a variety of data-depths (whole-genome/transcriptome sequencing, Fluidigm Biomark Dynamic Arrays, and targeted qPCR), as well as environmental DNA (eDNA) techniques, to examine the patterns of salmonid pathogen presence/absence in the Sacramento/San Joaquin River Delta system, as well as understand the host (Chinook salmon) genomic response to infection.

Hopkins Marine Station, Pacific Grove

My dissertation focused on small-scale patterns of natural selection in the rocky intertidal zone, kelp forest canopy, and a commercial fish species. I used transcriptomic analysis to examine both gene expression differences and structural DNA differences in gene sequences (single nucleotide polymorphisms, SNPs) at sub-meter scales in the California mussel (Mytilus californianus), whole-genome resequencing to understand patterns of population differentiation and the genic content of chromosomal inversions (possible 'supergenes') within Atlantic cod (Gadus morhua) in the Gulf of Maine, and DNA metabarcoding to look at depth and distance related changes in the benthic macro-/microinvertebrate settlement communities under kelp forest canopies in Monterey Bay, CA.

Manage and review data from contract testing laboratories. Build QC systems around testing and control of materials and finished product. Write, edit, and review SOPs, Method Transfer, and Method Validation documents.

hybridoma culture, ELISA assay development, antibody isolation, purification, and characterization (SDS-PAGE, Western Blot)

Contract through Kelly Temp Services, same job as before at this position. Plus method transfer completion, site shutdown activities, and documentation wrap-up.

Responsible for all aspects of international method transfer projects, including negotiation of responsibilities, creation of method transfer protocols, test scheduling, test data review, and final report generation for analytical methodsInitiated and led a cross-functional team including members from Development, Quality, Information Technology, and outside vendors to upgrade the validated chromatography data system (Agilent ChemStation and ChemStore)Managed method validation projects, including evaluation of existing older method validation reports against current practices, creation of method validation protocols, test data review, and final report generationAssisted preparation for and management of three FDA Audits, including a Pre-Approval Inspection, resulting in approval of a biopharmaceutical productManaged 5 employees, including developing all job responsibilities, training, and mentoring career development

Doctor Of Philosophy (Phd), Ecology And Evolutionary Biology

M.S., Biology - Conservation Genetics

Masters Thesis: Population Genetics of Dungeness Crab across Oregon, Washington, and British Columbia

B.S., Biological Sciences