• Successfully led the cell line engineering and molecular assays development team to support rAAV9 potency assays for Tay Sachs disease and Giant Axonal Neuropathy. • Developed and characterized in vitro disease models for Tay Sachs Disease and Giant Axonal Neuropathy.• Successfully established and operated two BSL2 laboratories in the fast-paced start-up environment, involved in acquisition of capital equipment and worked with CROs.

• Worked as a cell line engineering scientist to investigate cellular and molecular mechanisms for optimal recombinant Adeno-associated virus (rAAV) vector production. • Led the cell line development program based on CRISPR-mediated gene editing in various mammalian cell lines.• Involved in small molecules screening and vector engineering projects aimed at enhancing the rAAV vector production.

• Led a project focussed on Investigation of the function of erythrocyte CD55 in P. falciparum infection using CRISPR/Cas9 genome editing in hematopoietic stem cell, ex-vivo hematopoiesis and microscopic imaging techniques, resulting in a first author manuscript under preparation.• Investigated the role of P. falciparum acyl-CoA-binding protein in the parasite development by conditional knock down of the parasite proteins, resulting in a first author manuscript under preparation.• Optimized nucleofection technique to achieve improved cell viability and attained gene editing efficiency up to 90% in primary cells by using CRISPR/Cas9.• Investigated the Influence of the PIEZO1 rs572934641 (E756Del) polymorphism in malaria parasite growth.• Studied protein-protein interaction using AVEXIS, overlay assays, erythrocyte binding assays and mass spectrometry.• Employed Mammalian cell culture, stem cell culture, hybridoma culture and co-culture techniques to study host pathogen interactions.• Extensively employed Immunofluorescence assay, confocal microscopy and live cell imaging to investigate the role of host factors in parasite invasion.• Performed cell based growth assays and drug assays to investigate parasite invasion and growth phenotypes in wild type and mutant red blood cells.• Collaborated with Dr. Frans Kuypers’s group at Children’s Hospital Oakland Research Institute.• Trained other scientists and students on operation and troubleshooting of flow cytometer and also on sample prep and experimental design for multi-parameter flow cytometry application.• Trained and mentored > 4 undergraduate and graduate students on lab procedures to perform and troubleshoot experiments and work independently. •Guided experimental design, prioritized multiple project goals to meet deadlines and provided budget and resource oversight.

• Designed & implemented research projects to investigate host-pathogen interactions in P. falciparum infected erythrocytes, which led to the identification of > 2 dozen of novel protein-protein interactions and the development of Microsphiltration assay to study the deformability of protein-loaded erythrocyte ghosts.• Prepared plasmid libraries for in vitro expression of erythrocyte and parasite proteins.• Studied protein-protein interactions using Y2H, protein co-precipitation, split-luciferase assay, and mass spectrometry.• Employed Octet protein binding assay to study kinetics of protein-ligand binding.• Introduced and established malaria culture, parasite transfection and ghost loading assays in the lab, which led to the enhanced lab productivity.• Established SOPs for several laboratory protocols, minimizing waste and improving efficiency.• Collaborated with Mass spectrometry facility on-campus to identify erythrocyte cytoskeletal protein partners of malaria parasite proteins, which resulted in data for 2 manuscripts.• Acquired training in Malaria Experimental Genetics at Wellcome Trust Genome Campus, UK. Incorporated learned technologies in ongoing researches, contributed in laboratory improvement projects and increased their effectiveness. • Mentored a student for a summer research program, which led to a conference paper. Trained student standard laboratory practices, experimental design, data analysis, and how to explain conclusions.

-Instructed and supervised laboratory sessions.-Performed pre-lab lectures and lab preparations.-Ran interaction sessions for students.-Conducted pre-exam and post-exam review sessions.-Proctored examinations.-Graded quizzes, assignments, and exams.

-Taught medical microbiology to 350 undergraduate medical students at their first and second year of the course.-Designed laboratory-based experiments related to medical microbiology and trained the students. -Served as a consultant microbiologist for the college affiliated diagnostic lab with 30+ clinical sample per day.-Maintained training to keep staff skills up and decreased staff turnover.-Launched collaborative research projects on intestinal parasites with a hospital, a local school, community and a multi-discipline team within the college and and unveiled the status of parasitic infection in different populations.-Executed research projects on hospital acquired infection, Methicillin resistant Staphylococcus aureus and Mycobacterium tuberculosis-Reviewed 3 research proposals as an executive member of institutional review board.-Mentored 3 students for designing and executing research along with review and interpretation of the research data leading to 3 publications

-Taught medical microbiology to students pursuing "Dental assistant" course

-Worked on multiple studies on intestinal parasitosis and soil transmitted helminthiasis.-Worked on studies on enteric bacteria like Salmonella spp,, Vibrio spp,, Shigella spp., Campylobacter spp. etc.