Caitlin Macadino Email & Phone Number

Lexington, Massachusetts, United States

Gene Editing

Medford, Massachusetts, United States

1) High-Throughput Production Team dedicated to the manufacture of double-stranded RNA sequences of interest for crop protection* Routine operation of Hamilton HamSTAR liquid handler system* DNA and RNA sizing with Advanced Analytical fragment analyzer* Process data analytics associated with manufactured sequences* Write sample reports for internal customers2) Strain Engineering Team responsible for the modification of E. Coli strains for use in GreenLight’s cell-free reaction platforms* Introduced genomic modifications into Escherichia coli using red recombineering with oligos or PCR products.* Constructed plasmids using restriction ligation, isothermal and Golden Gate assembly methods* Analyzed Sanger and Next-Generation Sequencing using VectorNTI and Geneious software* Employed molecular cloning techniques to transform strains for expression of recombinant proteins* Conducted induction studies to assess recombinant protein levels in expression strains and analyzed proteins via SDS-PAGE and Western blot.* Significantly contributed to the reduction to practice of Patent US9688977B2: ‘Engineered phosphoglucose isomerase proteins with a protease cleavage site’* Helped develop and reduce to practice a proprietary plasmid addiction system (co-inventor on pending patent)* Designed and carried out experiments to probe the capability of bacterial strains to produce custom chemicals. Participate in experiments to increase yields of dsRNA produced from DNA templates.* Designed oligos for sequencing, colony screening, cloning and strain modifications.* Lead coordination and implementation of regular monthly cleaning efforts in Metabolic Engineering lab space.Other GreenLight Roles:Associate Scientist: Jan 2015-Dec 2017Senior Research Associate: May 2012-Dec 2014Research Associate Mar 2010-May 2012

Teams responsible for the manufacture of protein and cell-based products used in drug discovery research.* Isolation and cryopreservation of fresh human hepaotcytes* On-call hepatocyte response team responsible for accepting human livers* Extraction of human and animal liver P450 microsomes from frozen tissue* Quality control of blended microsome products using HPLC and spectrophotometry* Microinjections of Xenopus laevis oocytes with transporter RNAs and quality control utilizing radio-labeled substrates

Master Of Science, Biology; Molecular Biotechnology

Related courses: Recombinant DNA Techniques Lab, Biology Process Projects Lab, Molecular Biology, Biomaterials and Tissue Engineering.

Bachelor Of Arts, Biology

Skills acquired: mammalian cell culture, aseptic technique, indirect immunofluorescence, PCR, and plasmid engineering for bacterial.