Calista Gilger Email & Phone Number

United States

Research Associate in product development at Cello Therapeutics with experience gained from initial product development, through animal studies, and approved for Phase I.• Assisted in GMP production of batches used for IND submission, which was approved in 1 month• Completed two batches of drug product in a GMP facility through fill finish• Built and acted as the company’s QA department including inventory receipt and disposition, instrument IQ/OQ/PM, procedure qualifications, batch record keeping, incident reporting, and batch release• Contributed to report writing for IND submission• Conducted stability tracking of the two batches since production• Developed a novel membrane coated, mRNA loaded LNP production process• Performed extensive research and trials in LNP formulation• Used T-junction and herringbone pattern microfluidics• Developed an assay to determine mRNA loading efficiency• Conducted in-vitro transfection experiments to support my product• Began patent writing with my method and supporting data• Familiarity gained with methods such as dissolution, DLS/Zeta-potential measurements, microfluidization/high energy homogenization, and TEM• Maintained familiarity with HPLC

San Diego, California, United States

Research Technician for product development at Cue Health with a focus on immunoassays• Performed analysis on a variety of instruments including but not limited to HPLC, SEM, FTIR, flow cytometer, and zetapotential• On HPLC I was the only operator developing and optimizing methods for purification of antibody conjugations, separation of contaminants in in-house buffers, and identification of unknown residues. This was performed on SEC, IEX and reverse phase columns. Fully mastered the operation of Agilent’s ChemStation software as well as maintenance of the instrument.• Ensured the quality of products in the development stage by ELISA assays• Researched, developed methods, performed experiments and presented progress on a weekly basis• Synthesized superparamagnetic iron oxide nanoparticles for antibody conjugation• Followed all GMP and GLP guidelines

San Diego County, California, United States

applications of organometallic chemistry to organic synthesis• ran iridium and copper-catalyzed bis-borylations of ketones and epoxide homologation reactions• analyzed and characterized substrates by NMR spectroscopy and HPLC• purified substrates by column chromatography and recrystallization• upheld lab safety practices including chemical waste disposal• managed glassware maintenance and chemical inventory • presented research

San Diego County, California, United States

• Oversaw up to $100,000 budget• met with administrators and event partners regarding board initiatives• utilized professional communication and planning skills to organize events promoting the inclusion of all students at USD.