Research Scientist
CurrentConducting research within the Payloads team on extracellular vesicles, RNA therapeutics and gene editingExperience includes:• Assay design and optimisation to characterise and quantify extracellular vesicle cargo at both the RNA and protein level• Utilising siRNAs to knockdown target genes and assessing potency through qPCR, western blot and reporter cell lines (Flow Cytometry)• Loading gene editing modalities into EVs and assessing genome edits in vitro•… Show more Conducting research within the Payloads team on extracellular vesicles, RNA therapeutics and gene editingExperience includes:• Assay design and optimisation to characterise and quantify extracellular vesicle cargo at both the RNA and protein level• Utilising siRNAs to knockdown target genes and assessing potency through qPCR, western blot and reporter cell lines (Flow Cytometry)• Loading gene editing modalities into EVs and assessing genome edits in vitro• Mammalian cell culture of adherant and suspension cell lines • DNA/RNA extraction (From cells and tissues), RT-qPCR, PCR, BCA, western blots, Jess Simple Western, bacterial transformation, plasmid purification, flow cytometry• Transient transfections in adherant and suspension cell lines• Extracellular vesicle production and purification• Tangential flow filtration (TFF) and chromotography: qEV (SEC) and AKTA • Writing SOPs and COSHH• Independent experiment design• Training colleagues in various assays• Data presentation• Recording data in an electronic lab book Show less
