Conducting research within the Payloads team on extracellular vesicles, RNA therapeutics and gene editingExperience includes:• Assay design and optimisation to characterise and quantify extracellular vesicle cargo at both the RNA and protein level• Utilising siRNAs to knockdown target genes and assessing potency through qPCR, western blot and reporter cell lines (Flow Cytometry)• Loading gene editing modalities into EVs and assessing genome edits in vitro•… Show more Conducting research within the Payloads team on extracellular vesicles, RNA therapeutics and gene editingExperience includes:• Assay design and optimisation to characterise and quantify extracellular vesicle cargo at both the RNA and protein level• Utilising siRNAs to knockdown target genes and assessing potency through qPCR, western blot and reporter cell lines (Flow Cytometry)• Loading gene editing modalities into EVs and assessing genome edits in vitro• Mammalian cell culture of adherant and suspension cell lines • DNA/RNA extraction (From cells and tissues), RT-qPCR, PCR, BCA, western blots, Jess Simple Western, bacterial transformation, plasmid purification, flow cytometry• Transient transfections in adherant and suspension cell lines• Extracellular vesicle production and purification• Tangential flow filtration (TFF) and chromotography: qEV (SEC) and AKTA • Writing SOPs and COSHH• Independent experiment design• Training colleagues in various assays• Data presentation• Recording data in an electronic lab book Show less

Working on protein and cell-based immunoassays for client projects.Responsibilities include:• Project leader of the ProStorm Cytokine Release Assay. Responsible for planning assays, leading lab work and writing project reports for clients within tight deadlines• Planning, scheduling and executing various assays within the cellular analysis services team• Writing SOPs and project reports for clients• Data analysis• Running T cell… Show more Working on protein and cell-based immunoassays for client projects.Responsibilities include:• Project leader of the ProStorm Cytokine Release Assay. Responsible for planning assays, leading lab work and writing project reports for clients within tight deadlines• Planning, scheduling and executing various assays within the cellular analysis services team• Writing SOPs and project reports for clients• Data analysis• Running T cell proliferation assays and ELISpots• Assay optimisation• Preparing and qualifying reagents prior to their use in assays• Isolating PBMCs from whole blood• Lab and equipment maintenance Show less

• Serving customers at the checkouts• Taking in deliveries and restocking shelves• Coordinating work effectively in teams of various sizes• Gold Customer Service Award

• Cloned gene variants into GFP constructs.• Transiently tranfsfect GFP constructs into HeLa cells.• Carried out western blots and immunocytochemistry to determine which isoform was being expressed and how this altered subcellular localisation. • Presented results in lab meetings. • Summarised findings in a report at the end of the project.