Camille Johnston Email & Phone Number

Copenhagen, Capital Region, Denmark

Post doc in the Section for Veterinary Virology at SSI. My projects are part of the EU4Health DURABLE project. In short I am expanding our capabilities at identifying veterinary virological pathogens, and looking into the variants in the virus subpopulations.

Copenhagen, Capital Region, Denmark

Post doc in the Section for Veterinary Virology at SSI. My project is part of the African Swine Fever pathogenesis and immune responses in Resistant And Susceptible Hosts (ASF-RASH) funded by ICRAD. In short I am looking into the virus subpopulations within African Swine Fever Virus (ASFV) infected pigs.• Developing a deep sequencing strategy for investigation of virus variants within ASFV infected pigs, by use of overlapping long PCRs (10-13 kb), covering almost the… Show more Post doc in the Section for Veterinary Virology at SSI. My project is part of the African Swine Fever pathogenesis and immune responses in Resistant And Susceptible Hosts (ASF-RASH) funded by ICRAD. In short I am looking into the virus subpopulations within African Swine Fever Virus (ASFV) infected pigs.• Developing a deep sequencing strategy for investigation of virus variants within ASFV infected pigs, by use of overlapping long PCRs (10-13 kb), covering almost the entirety of the ASFV genome• Developing and implementing bioinformatic pipelines for sequence analysis • Developing python and R scripts for data analysis and data visualization• Variant analysis and haplotyping of viral subpopulations• Perform NGS using Oxford Nanopore and Illumina MiSeq Show less

Copenhagen Area, Capital Region, Denmark

• Developing in-house python and R scripts for data analysis and data visualization• Developing shiny apps for data analysis and visualization for customer use• Instituting long read sequencing applications with Oxford Nanopore Technology (ONT)• Establishing various bioinformatical pipelines for short and long read sequencing (Illumina/ONT) • Project responsible for improvement projects in R&D• Responsible for collaboration with externals on GUI development for Xdrop Sort

Herlev

• Establish a pipeline for bioinformatic analyses• Analysis and interpretation of data

Lindholm, Kalvehave

• Development of diagnostic methods• Analysis and interpretation of data• Sequencing • Composing manuscripts for publicationSpecifically, I worked with establishing cell culture systems and development of immunostaining tests (IPT) for detection of atypical porcine pestivirus (APPV) antibodies as part of a CoVetLab project, as well as full-length sequencing of bovine viral diarrhea virus (BVDV) by random NGS. I also created full-length cDNA of APPV for targeted NGS.

I helped arrange social events for the PhD students at DTU Vet, to encourage and strengthen the bonds between peers.

Lindholm, Kalvehave

• PhD Thesis: ‘Investigation of the genetic basis for virus tropism and virulence of classical swine fever virus’• Analysis and interpretation of data • Supervision of students and laboratory technician interns in the laboratory • Project management • Composing manuscripts for publication• Utilized various molecular methods, such as whole-genome sequencing of full-length viral cDNA, various PCR’s, purification techniques, molecular cloning, site-directed mutagenesis… Show more • PhD Thesis: ‘Investigation of the genetic basis for virus tropism and virulence of classical swine fever virus’• Analysis and interpretation of data • Supervision of students and laboratory technician interns in the laboratory • Project management • Composing manuscripts for publication• Utilized various molecular methods, such as whole-genome sequencing of full-length viral cDNA, various PCR’s, purification techniques, molecular cloning, site-directed mutagenesis, transformation, transfection, cell culture, immunostaining techniques, and sequencing. PhD in molecular virology studying classical swine fever (CSFV), a pestivirus belonging to the Flaviviridae family. I worked with optimization of full-length cloning (12.3 kb) of RNA viruses, using In-Fusion and TOPO-XL2 cloning, in order to haplotype and map virus subpopulations. The E2 glycoprotein of CSFV is responsible for the viral binding to host cells, and my focus has been on a specific motif herein (aa 761-764). Mutants were created by site-directed mutagenesis and tested in vivo in pigs. Tissues from pigs were analyzed by NGS to reveal differences in quasispecies composition . Mutants were also tested in vitro in different cell lines to reveal their role in cell specificity. Show less

5 month's research stay at Hokkaido University, Sapporo, Japan, as a part of PhD.

Hillerød

• Receive, handle and document release samples submitted to QC lab in LIMS• Handle, distribute and aliquoting of LSM samples • Review and document stability samples• Working under GMP

Helsingør, Danmark

Research in prophages of Flavobacterium psychrophilum and Vibrio anguillarum

Nagoya, Aichi, Japan

Exchange student at Nagoya University. Did a project with cyanophages isolated from rice fields.Taught Danish at a language school.

Doctor Of Philosophy - Phd, Molecular Virology

My thesis topic was investigation of the genetic basis for virus tropism and virulence of classical swine fever virus (CSFV), a pestivirus.

Cand.Scient, Biochemistry

Master thesis: ‘Phenotypic and genomic characterization and attempted curing of prophages in Flavobacterium psychrophilum, a fish pathogen’.

Bachelor Of Science (B.Sc.), Biochemistry

Bachelor projekt: 'Induktion og karakterisering af profager i Flavobacterium psychrophilum, et Fiskepatogen'

Htx, Chemistry A And Biology B