Thomas Cardon Email & Phone Number

San Diego Metropolitan Area

Cleveland, Ohio, United States

• Supported internal groups (Stability, Pre-clinical, and Assay Development) as well as external contract research organizations (CRO) through collaboration with purification team in order to produce hundreds of insulin analogs.• Worked closely with the expression team to support their efforts in screening new clones, optimizing yields and enzyme activities, and assisting in troubleshooting their process.• Assisted with early-stage process development and good manufacturing practices (GMP) of production involving one insulin analog that supported an investigational new drug (IND) application for the food and drug administration (FDA).• Researched, developed and optimized upstream/downstream protein purification, digestion, and filtration protocols:- Screened new yeast clones using LC-MS to confirm identify of target protein.- Analytical HPLC/UPLC to quantitate yield and purity of target protein and troubleshoot protocols.- Upstream protein purification using IMAC, HIC, and preparative RP HPLC (1mg – 2g scale per batch).- Downstream protein purification through utilization of semi-preparative and preparative RP HPLC.- Tangential-flow filtration (TFF).- Lyophilization.- Enzymatic digestions (trypsin, endo Lys-C, and carboxypeptidase B) of insulin analogs.- Endo Lys-C activity assay.• Characterized insulin analogs by using HPLC/UPLC, LC-MS, SDS-Page, and UV-vis spectroscopy.• Worked with supervisor to propose, design, and perform early-stage process development experiments for three protocols (IEX, TFF, and trypsin digestion of insulin analog).• Assisted in writing and/or editing SOPs for GMP engineering run for IEX, TFF, preparative RP HPLC, trypsin digestion, and lyophilization protocols.• Troubleshot, fixed, and modified various instruments including: - TFF (cassettes & pumps)- Analytical and preparative HPLC instruments- Lyophilizers (valves, manifold, pumps)- Solid-phase peptide synthesizer

Cincinnati, Ohio, United States

• Specialized in the 505(b)(2) regulatory pathway:- Searched published scientific literature and publicly available documents from the FDA to support the safety and efficacy of a client’s drug product using 505(b)(2) guidelines.- Assisted in writing safety and efficacy sections for 4 INDs and 2 NDAs in CTD format.- Wrote style guide for client and internal use in writing FDA applications.

Columbus, Ohio, United States

• Over-expressed iNOS in E. coli.• Purified iNOS using Ni-IMAC, ion-exchange, affinity, and size-exclusion chromatography.• Studied enzyme kinetics of nitric oxide, superoxide, and peroxynitrite production from iNOS using spin-trap EPR spectroscopy, radiolabeled assays, UV-Vis spectroscopy, and NO gas-sensing electrodes.• Performed high-pressure homogenization of E. coli cells.• Trained colleagues on the operation of the homogenizer.

Oxford, Ohio, United States

• Served as SME for solid-phase peptide synthesis, EPR Spectroscopy, and Lyophilization protocols:- Designed peptide synthesis lab and maintained, troubleshot, and fixed instrumentation.- Wrote user’s manual for the operation and troubleshooting of the automated peptide synthesizer.- Developed and wrote protocols to synthesize, process, and purify peptides for colleagues’ research projects.- Trained 13+ colleagues on instrumentation; how to synthesize, process, and purify peptides using these protocols.- Acquired and analyzed EPR spectra, wrote and organized methods/results for use by colleagues in research articles.• Maintained and fixed peptide synthesizer, EPR spectrometer, lyophilizer, table-top centrifuge, preparative FPLC, and rotary evaporative instruments and pumps.• Developed novel method using Spin-labelled EPR spectroscopy to study the structure, dynamics, and function of membrane peptides/proteins embedded into magnetically-aligned bicelle membrane disks.• Prepared samples for and analyzed results of solid-phase EPR and NMR spectra.• Developed, wrote, and performed protocols for reverse-phase HPLC purification of synthesized membrane peptides.• Acquired/analyzed results from mass spectrometry (MALDI-TOF, capillary electrophoresis MS, LC-MS, and MS/MS).• Prepared samples for and performed lyophilization of membrane peptides.• Prepared bicelle membranes with incorporated membrane peptides.• Served as a teaching assistant for introductory chemistry labs for 1.5 years.

Salt Lake City, Utah, United States

• Purified transcobalamin II from raw cows’ milk by chilling/centrifugation and affinity chromatography.• Filtered samples using diatomaceous earth and diafiltered/ultrafiltered samples using dialysis tubing.• Characterized proteins using SDS-Page gels, BCA assay, and UV-Vis spectroscopy.

Logan, Utah, United States

• Overexpression of ferritin in T. thermophila and baculovirus expression systems.• Protein purification of heat-tolerant ferritin (from T. thermophila) using selective protein precipitation with heat.• Protein purification of ferritin (from baculovirus) using IEX.• Diafiltration using dialysis tubing and Amicon stirred cells.• Characterized proteins using UV-Vis, SDS-page, BCA assay.

Postdoctoral Research, (Davis Heart & Lung Research Institute)

Phd, Biochemistry & Chemistry

(Department of Chemistry & Biochemistry)

Bachelor Of Science - Bs, Chemistry (Emphasis In Biochemistry)

(Department of Chemistry)