Thomas Cardon Email & Phone Number

San Diego Metropolitan Area

Cleveland, Ohio, United States

• Supported internal groups (Stability, Pre-clinical, and Assay Development) as well as external contract research organizations (CRO) through collaboration with purification team in order to produce hundreds of insulin.
• Worked closely with the expression team to support their efforts in screening new clones, optimizing yields and enzyme activities, and assisting in troubleshooting their process.
• Assisted with early-stage process development and good manufacturing practices (GMP) of production involving one insulin analog that supported an investigational new drug (IND) application for the food and drug.
• Researched, developed and optimized upstream/downstream protein purification, digestion, and filtration protocols:- Screened new yeast clones using LC-MS to confirm identify of target protein.- Analytical HPLC/UPLC to.
• Characterized insulin analogs by using HPLC/UPLC, LC-MS, SDS-Page, and UV-vis spectroscopy.
• Worked with supervisor to propose, design, and perform early-stage process development experiments for three protocols (IEX, TFF, and trypsin digestion of insulin analog).

Cincinnati, Ohio, United States

• Specialized in the 505(b)(2) regulatory pathway:- Searched published scientific literature and publicly available documents from the FDA to support the safety and efficacy of a client’s drug product using 505(b)(2).

Columbus, Ohio, United States

• Over-expressed iNOS in E. coli.
• Purified iNOS using Ni-IMAC, ion-exchange, affinity, and size-exclusion chromatography.
• Studied enzyme kinetics of nitric oxide, superoxide, and peroxynitrite production from iNOS using spin-trap EPR spectroscopy, radiolabeled assays, UV-Vis spectroscopy, and NO gas-sensing electrodes.
• Performed high-pressure homogenization of E. coli cells.
• Trained colleagues on the operation of the homogenizer.

Oxford, Ohio, United States

• Served as SME for solid-phase peptide synthesis, EPR Spectroscopy, and Lyophilization protocols:- Designed peptide synthesis lab and maintained, troubleshot, and fixed instrumentation.- Wrote user’s manual for the.
• Maintained and fixed peptide synthesizer, EPR spectrometer, lyophilizer, table-top centrifuge, preparative FPLC, and rotary evaporative instruments and pumps.
• Developed novel method using Spin-labelled EPR spectroscopy to study the structure, dynamics, and function of membrane peptides/proteins embedded into magnetically-aligned bicelle membrane disks.
• Prepared samples for and analyzed results of solid-phase EPR and NMR spectra.
• Developed, wrote, and performed protocols for reverse-phase HPLC purification of synthesized membrane peptides.
• Acquired/analyzed results from mass spectrometry (MALDI-TOF, capillary electrophoresis MS, LC-MS, and MS/MS).

Salt Lake City, Utah, United States

• Purified transcobalamin II from raw cows’ milk by chilling/centrifugation and affinity chromatography.
• Filtered samples using diatomaceous earth and diafiltered/ultrafiltered samples using dialysis tubing.
• Characterized proteins using SDS-Page gels, BCA assay, and UV-Vis spectroscopy.

Logan, Utah, United States

• Overexpression of ferritin in T. thermophila and baculovirus expression systems.
• Protein purification of heat-tolerant ferritin (from T. thermophila) using selective protein precipitation with heat.
• Protein purification of ferritin (from baculovirus) using IEX.
• Diafiltration using dialysis tubing and Amicon stirred cells.
• Characterized proteins using UV-Vis, SDS-page, BCA assay.

Postdoctoral Research, (Davis Heart & Lung Research Institute)

Phd, Biochemistry & Chemistry

(Department of Chemistry & Biochemistry)

Bachelor Of Science - Bs, Chemistry (Emphasis In Biochemistry)

(Department of Chemistry)