Development of therapeutic and reagent monoclonal antibodies using hybridoma technologyAnimal immunization strategy optimization and designDesign and execution of large high throughput primary antibody screening assays by using multi-color FACS, ELISA, and cell killing assaysProficient with FlowJo and Prism Execution and optimization of robotic high throughput screening for selection of drug candidatesSupported discussion regarding antigen source and functional screening assaysSingle-cell discrimination and sorting via FACSTransient Transfection for; protein production, cell line QC, plasmid construction QCExperience with safe animal handling procedures and in vivo work

Hybridoma Development for the Antibody Discovery department

Hybridoma Development for the Antibody Discovery group

In vitro generation and growth of stable hybridoma cell lines for production of monoclonal antibodies, along with numerous established research cell lines from which proteins are extracted.mammalian cell culture, differentiation assays, monoclonal antibody development, ELISA, Western Blot

Gathering real time fishery management data for NOAA, NMFS, and any other fishing regulatory divisions within Alaskan fishing management. Living and working aboard U.S.-flagged commercial fishing vessels operating in the Bering Sea, North Pacific, and Gulf of Alaska.