Design and purify antibodies to detect RCAN1 isoforms present in brain. Perform western blots to quantify isoform expression in normal and Alzheimer brain samplesUse RT PCR to construct RCAN1 probes for use in Northern Blot analysis of expression in brainExamine RNA isoform expression in various cell types in human brain tissue using combined immunocytochemistry and in situ hybridization. Use a tet-off system in cell culture to examine the effect of RCAN1-1 on GSK-3 and tau phosphorylation levels and to measure the effects of oxidative and calcium challengesWrite papers and prepare posters for publication or to present results of experimental data.

Instruct students on proper laboratory techniques, data collection and preparation of lab reports while maintaining a safe environment Facilitate discussions to engage students on course material and reinforce learning. Evaluate and monitor student progress on coursework and exams

Identify diseases of small fruits both visually and microscopically. Perform fungicide efficacy trials, including resistance studies.Maintain laboratory chemical inventories and MSDS files.Prepare solutions and supplies for experiments