Céline Leclercq Email & Phone Number

Käerjeng, Lu

Bascharage, Luxembourg, Luxembourg

ERIN Department / BEAP Biotechnologies and Environmental Analytics Platform.My role : • Protein extraction from different tissues, organisms, cell culture. Membrane proteins enrichment. Cell wall proteins extraction development.• Sample fractionation using preparative LC, 1D, 2D and 2D DIGE gels electrophoresis, Offgel fractionation, separation of peptides or proteins using Nano HPLC to MS, western blotting.• Automatic handle workstation for picking, digestion… Show more ERIN Department / BEAP Biotechnologies and Environmental Analytics Platform.My role : • Protein extraction from different tissues, organisms, cell culture. Membrane proteins enrichment. Cell wall proteins extraction development.• Sample fractionation using preparative LC, 1D, 2D and 2D DIGE gels electrophoresis, Offgel fractionation, separation of peptides or proteins using Nano HPLC to MS, western blotting.• Automatic handle workstation for picking, digestion, extraction, spotting…• For the identification of proteins, relative and absolute quantitation, PTM isolation and library creation with SWATH acquisition: liquid chromatography and high resolution mass spectrometry (LC-MS); practical experience in the use of NanoLC-425_TripleTOF™6600 (micro and nano mode), SWATH technology, NanoLC-2D_TripleTOF™5600+, MALDI-TOF/TOF™ 5800, Ultimate 3000 nanoLC_NSI-LTQ-Orbitrap Elite™, Probot LC packing, MALDI-TOF/TOF™ 4800, Sunchrom fraction collector, Q-TOF and Orbitrap Velos™. • Bioinformatics: database research, validation and quantification (gel based, label free or labelling...), SWATH analysis.Used datatreatment softwares: Mascot Daemon, Progenesis QIP, PeakView, Protein pilot, TPP, Decyder, Scaffold, MaxQuant, Proteome discoverer...Device softwares: Eksigent, Analyst, 4000 series explorer, Image Quant, Xcalibur, Chromeleon.• For the department, technical and logistical support work (stock management, chemical management, order management), servicing and maintenance of common equipments. Show less

Grenoble

Proteomic engineer on EDyP-Service platform.Research project: “The analysis membrane fractions analysis of oligodendroglial progenitors and oligodendrogliomas and development of strategy for label-free quantification”.• Research project on brain tumours specifically oligodendroglioma ;• Proteomics analysis by high throughput mass spectrometry: using a Q-TOF and an LTQ-OrbiTrap coupled to two liquid chromatography Ultimate-3000 nanoLCs in parallel ;• Establishment of… Show more Proteomic engineer on EDyP-Service platform.Research project: “The analysis membrane fractions analysis of oligodendroglial progenitors and oligodendrogliomas and development of strategy for label-free quantification”.• Research project on brain tumours specifically oligodendroglioma ;• Proteomics analysis by high throughput mass spectrometry: using a Q-TOF and an LTQ-OrbiTrap coupled to two liquid chromatography Ultimate-3000 nanoLCs in parallel ;• Establishment of the relative quantification technique by Spectral Counting in comparison with the index of protein abundance emPAI. Show less

Ustl Lille - Villeneuve D'Ascq

Characterization of biomolecules by high mobility transistor - MEMS, BioMEMS

Montréal

Research training in Microbiology Bioremediation lab, NRC-CNRC-Biotechnology Research Institute – Montreal Quebec. • Biodegradation of contaminants in soils and groundwater (environmental pollutants).• Validation of oligonucleotides microarray• Designing a microarray targeting the variants of alkB genes involved in alkane degradation pathways in many microorganisms.Techniques used: total soil DNA extraction, DNA microarrays , labelling and hybridization genomic… Show more Research training in Microbiology Bioremediation lab, NRC-CNRC-Biotechnology Research Institute – Montreal Quebec. • Biodegradation of contaminants in soils and groundwater (environmental pollutants).• Validation of oligonucleotides microarray• Designing a microarray targeting the variants of alkB genes involved in alkane degradation pathways in many microorganisms.Techniques used: total soil DNA extraction, DNA microarrays , labelling and hybridization genomic DNA of strain "pure" and total DNA of soil, detection limit, sensitivity, data analysis, general bioinformatics for genomics and proteomics. Show less

Lille

Research training on “Cloning specific proteins of mammalian brain involved in the phenomena of plasticity of the median eminence”. Techniques used : ADN extraction, purification, PCR, RT-PCR, cloning, sequencing, linearisation.

Marquion

Harvest 2003 - 2005 - 2006Analysis of various seeds from agriculture: calculation of impurities, statement of humidity, PS and protein content of wheat and barley, Hagberg falling number (alpha-amylase activity), sampling, mycotoxin risk.

M2 Biologie Et Biotechnologies, Protéomique Et Physico-Chimie Du Vivant

Activities and Societies: Proté'Asso 2003-2007 IUP Génomique et Protéomique specialisé Biotechnologies et Bio-industrie (licence, master1).

1Ère Année, Médecine

1ère année de Médecine