Chao Chen Email & Phone Number

St. Louis, Missouri

Fort Collins, Colorado Area

• Successfully designed SDS‐PAGE gel experiments along with western blot to illuminate the mechanism how a protein conjugated with GTP
• Applied in-gel digestion for LC-MS samples, to pinpoint the modification site of a protein
• Investigated an enzymatic reaction on in-vitro transcribed RNAs using 32P as a radioactive tracer

Charleston, SC

• Research on liposomal drug delivery:
• Established the platform to explore liposomal drug delivery against brain tumors, covering the methodologies to fabricate liposomes, to incorporate drugs, to purify liposomal drugs using SEC-HPLC and to analyze their.
• Pharmacokinetic study of liposomal doxorubicin, employing novel reliable analytical methods based on RP-HPLC or a plate reader with fluorescence measurement, guiding a collaboration project with a pediatric brain tumor.
• Already published in Current Neuropharmacology and Critical Reviews™ in Biomedical Engineering, and expecting another two

Bloomington, IN

• Studied how viral proteins assemble into capsids and interact with host components:
• Laid the foundation for >3 research projects in Prof. Adam Zlotnick’s lab by developing the protocols for cell culture expression and the subsequent FPLC purification (with SEC, IEX and IMAC columns) of recombinant.
• Studied the interaction of HBV to IMPβ through particularly-designed SEC-FPLC experiments, and consequently characterized the binding moiety, electrostatic nature and some other details at the molecular level, which.
• Employed IMAC-FPLC, centrifugation and SDS-PAGE in innovative approaches to characterize and quantify the interaction between hepatitis B virus (HBV) and the kinase SRPK, shed light on HBV’s life cycle and published on.
• Created a DLS methodology to quantify viral capsid assembly, which illuminated the molecular mechanism how RRV, the cause for Ross River fever, was constructed, illustrated a new application of DLS for virology study.
• Frequent collaboration with virologists, analytical chemists and a cryo-EM expert by performing or proposing experiments, providing particular samples and suggesting data interpretation, which helped to effectively.

Bloomington, IN

• Doctoral research on the novel hybrid system of gold nanoparticle-viral capsid and pure viral capsids:
• Created the nano-system of viral capsids encapsulating gold nanoparticles, which supplied a good model for studying virus assembly, showed the direction how to package drugs into viral capsids for delivery, provided a.
• Developed original methodologies involving fluorescence spectroscopy, electrophoresis, a customized laser setup and DLS to investigate viral capsid assembly, which illuminated details of the process at the molecular.
• 6 publications in peer-reviewed journals including the high-impact Nano Letters and Journals of the American Chemical Society, with hundreds of citations

Beijing City, China

Bachelor thesis project: AFM study of polymer crystallization with confinement in two-dimensions