Florence Chardon Email & Phone Number

Seattle, Washington, United States

Computational biology team lead at the Seattle Hub for Synthetic Biology. We are leveraging DNA Typewriter technology to build a prime editing recorder mouse. I write and employ computational pipelines to process and analyze next-generation sequencing data.

Greater Seattle Area

Graduate student in Dr. Jay Shendure's lab and co-advised by Dr. Jay Shendure and Dr. Lea Starita. Research focused on developing novel multiplexed, massively parallel, high throughput single-cell and bulk genomics technologies. http://shendure-web.gs.washington.edu/

South San Francisco, California

• Research Associate in Protein Analytical Chemistry. Mass spectrometry method development and analysis for monoclonal antibodies, bispecific antibodies, and Fab fragments.Completed a 2-year rotational program in four.
• Developed and validated an analytical assay for antibody and antibody-drug conjugate (ADC) characterization for PC/PV (Process Characterization/Process Validation) 2) Early Stage Cell Culture
• Identified mitigation strategies for two unique runaway lactate issues seen in two stable cell lines through fermentation experiments 3) Purification Development
• Identified beta glucan clearance strategies to meet impurity limits for ocular product
• Succesfully brought an out-sourced asasy in-house and demonstrated accuracy and comparability; saved the department tens of thousands of dollars and weeks of time savings 4) Drug Delivery
• Worked on conjugation chemistry of Fab-PEG constructs

Berkeley, CA

• Campus liaison between Genentech and UC Berkeley
• Educate students on the UC Berkeley campus about opportunities available at Genentech for current students as well as recent graduates
• Organize events for students to learn more about the company
• Work with Genentech College Programs in Human Resources to bring new research interns to the company

South San Francisco, CA

• Intern in Genentech’s Research and Early Development (gRED) Small Molecule Process Chemistry/Drug Discovery department
• Worked with thin-layer and automated column chromatography for the separation of drug and drug-like molecules
• 40 hours a week for the duration of 11 weeks
• Published a paper on this project in the Royal Society of Chemistry's Green Chemistry journal; Publication date: July 2014

Fischer Laboratory, College Of Chemistry, UC Berkeley

• Synthesized organic precursor monomers for utilization in ring-opening alkyne metathesis polymerization (ROAMP) for the assembly of graphene nanoribbons (GNRs)
• Researched and designed new synthetic routes to synthesize highly-strained, conformationally unfavored molecules
• Attempted to discover a high-yielding method to scale up the synthesis of these monomers from less than 500mg of product to over one gram of product
• 20-30 hours a week

Earth And Planetary Sciences Department

• Assisted Professor Robert Rhew in research projects on atmospheric trace gases and how they catalyze ozone layer destruction
• Operated a gas chromatography-mass spectrometry (GC-MS) instrument
• Measured ethylene gas emissions with a Laser Photo Acoustic Spectroscopy Instrument (LPAS)
• Performed methyl halide extractions on various plants
• 10 hours a week

Doctor Of Philosophy (Phd) In Genome Sciences

Bachelor Of Science (B.S.), Chemistry - College Of Chemistry

Activities and Societies: Club Volleyball

Biochemistry And Molecular Biology

• Semester Abroad in Bordeaux, France

Education record