Charles Hay Email & Phone Number

Milwaukee, WI, US

San Diego, California, US

Coordinated with CROs to plan and execute IND-enabling pharmacokinetic and toxicology studies Analyzed, translated, presented pharmacokinetic and toxicology study data from CROs to the company in non-scientific terminology

WI, US

Worked with vendors to order and establish a new neuro oncology laboratory Utilized lab techniques, such as: tissue culturing of primary and immortal glioblastoma and breast cancer cells line, 5 color flow cytometry, immunoassays, ELISAs, and mini preps Worked with PI and collaborators to write multiple grants, and worked with department administration to.

WI, US

Developed protocols and methods for the development of chimeric antigen receptor T (CAR-T) cell therapies to treat multiple myeloma and various lymphoma cancersDesigned and developed 4 novel, single chain bispecific antibodies capable of reducing manufacturing costs and time by more than 60%, and significantly increasing potency compared to the lab’s.

• Develop assay protocols for monoclonal antibodies and gene therapies, manage the lab via ordering, maintenance, and record keeping, and mentor more than 14 junior and pre-candidacy PhD students in the lab and classroom
• Perform molecular cloning and use behavioral & biodistribution assays, tail bleeds, and subcutaneous (s.c.) dosing in mouse models
• Analyze western-blots using LICor ImageStudio, use Serial Cloner in plasmid construct design, employ Labguru Electronic Lab Notebook (ELN) for accurate record keeping, and analyze data using Graphpad Prism
• Designed, cloned, and characterized 3 iterations of an anti-methamphetamine (meth) monoclonal antibody-based gene therapy to aid in meth abuse recovery
• Increased the circulating concentrations of final scFv-Fc fusion protein 30-fold over original construct design; resulted in prevention of psychostimulant effects of meth at high doses (10 mg/kg, s.c.) in mouse models
• Presented data from project in 24 poster presentations, 8 seminars, and 2 first-author & 2 co-author publications

• Developed reproducible, reliable, and accurate assay protocols, analyzed the data using Graphpad Prism, and mentored 3 summer and rotating students in assay design and theory
• Performed aseptic tissue culturing & transfection in human cell lines and immunoassays (flow cytometry, ELISA, immunoprecipitation, and western-blots) to identify 2 novel auto-antigens/biomarkers in patients with.

Mount Vernon, IA, US

• Performed molecular cloning & Sanger sequencing and developed phylogenetic tree models in order to differentiate species of fire coral showing phenotypic plasticity; managed and mentored 2 junior lab students
• Presented data in poster presentations at 4 local conferences and contributed to 3 professional publications

Doctor Of Philosophy (Phd), Pharmacology And Toxicology

Master Of Science (M.S.), Pathology And Microbiology

Bachelors Of Special Studies (B.S.S.), Biochemistry, Molecular Biology, And Mathematics