Chetna Dwivedi Email & Phone Number

Rancho Cordova, California, United States

San Jose

San Francisco Bay Area

Assay development to drive mechanism of action in the field of novel drug target. Perfromedrobotics fluids using Qiagen platform , automated drug dosing using Tecan, automated analysis of live cell images using Incucyte S3 software, 96 well plate reporter assay, Protein quantification using Bradford assay, Protein analysis using automated western analysis jes wes( protein simple), traditional western blotting, maintaining various cancerous cell lines both adherent and suspended.

San Francisco Bay Area

DNA synthesis, amplification, and cloning, Gibson Assembly , protein engineering via site directed mutagenesis using Benchling software, Genome complier software and quantification using Image J processing programme. DNA concentration using nano drop and spectrophotometer. Maintenance and manipulation of various mammalian tissue culture cell lines.Transfection and transduction on cancerous cell lines, Reporter assay using 96 well plate. Cell imaging via standard fluorescence microscopy.Techniques used routinely such as traditional Western Blotting, ELISA, Affinity chromatography, SDS-PAGE. Routine maintenance of lab work such as timely buffer and reagent preparation, calibration of instruments etc.

Maharashtra, India

Analysis of Flow Cytometry patient data of Clinical Trials using BD Facs-Diva software. Data Management: Collating data from global sites, data managing and uploading to the sponsor sites as per protocols.

Maharashtra, India

Expertise in all aspects of flow cytometry including Instrument set-up, daily quality control using (flow-check beads, CST beads, 8 color peak beads). Sample processing (lymph nodes,tissue processing, body fluids, bone marrow, peripheral blood, FNAC).Acquisition of samples on 10 color Beckman coulter Navios II, BD Facscanto II, 13 color BC (Cyto-flex) platform. Data analysis through kaluza software for Beckman instruments, facsdiva and flow Jo for BD instrument.Performed an independent research project on a characterization of ZAP70, a marker associated with poor prognosis for Chronic lymphoproliferative leukemia.Study on correlation of CD25 in AML patient as a prognostic marker.

New Delhi Area, India

Trained in aseptic techniques for maintaining multiple cell lines (adherent and suspension). Characterized various culture parameters e.g. doubling time, cryopreservation and revival of cell lines, media preparation. Pre-clinical testing of Dabur skin products for allergic sensitivity in the mouse model. Trained in animal handling and experiment design (animal adaptation, randomization, set up of placebo) and primary culture. Assays performed included Local lymph node assay (LLNA) for measuring lymph node thickness, an indicator of an allergic reaction; measurement of cell proliferation using BrdU incorporation.

Master Of Science - Ms, Biosciences, A

Activities and Societies: An active participant in cancer genomics laboratory.Synthesis and characterization of nanoparticle.

Post Graduate Diploma, Molecular And Biochemical Technology

Hand on experience in biophysical, immunological and recombinant dna technological assays

Bachelor Of Science - Bs, Life Sciences

Activities and Societies: Member of Genesis society

Summer Internship, Biochemistry

Activities and Societies: Department of BiochemistrySummer Research Project in Department of Biochemistry at Guru Gobind Singh.