Chi (Aimee) Zhao, Ph.D.

Chi (Aimee) Zhao, Ph.D. Email and Phone Number

Automation Scientist | Biomedical Engineer @ 10x Genomics
Chi (Aimee) Zhao, Ph.D.'s Location
Palo Alto, California, United States, United States
Chi (Aimee) Zhao, Ph.D.'s Contact Details

Chi (Aimee) Zhao, Ph.D. work email

Chi (Aimee) Zhao, Ph.D. personal email

n/a

Chi (Aimee) Zhao, Ph.D. phone numbers

About Chi (Aimee) Zhao, Ph.D.

The primary efforts of my Ph.D. research are directed towards engineering chimeric transmembrane proteins for both translational and fundamental studies. Specifically, the goal of my translational research is to develop cell-derived biomaterials for specific and efficient drug delivery. To fulfill this aim, I focused on 1) engineering multifunctional transmembrane proteins for targeting cell-derived vesicles to cancer cells, and 2) decorating these vesicles with high concentrations of transmembrane channel proteins to facilitate the unloading of macromolecular drugs directly into the cytoplasm, maximizing their efficacy. Using the same chimeric proteins developed in these two projects, my research in fundamental science focuses on understanding the underlying mechanism of receptor uptake by endocytosis, an essential pathway for drug delivery vehicles to enter cells.Working as an experimentalist, I have 5+ years of wet lab experience with proficiency in mammalian cell culture, fluorescence microscopy, flow cytometry, molecular cloning, lentiviral transfection, gene editing, and protein purification. Working as a data analyst, I have performed complex analyses to microscopy data collected from mammalian cells using MATLAB. Further, I have 5+ years of experience in using Adobe Photoshop and Illustrator to create illustrations for my publications. My original research so far has been published as 7 first-authored or co-authored peer-reviewed papers with 3 more to come. During my Ph.D., I have mentored 8 undergraduate researchers, and 2 junior graduate researchers. My experience in being a team leader has taught me how to adapt to and foster a collaborative environment. I am looking to leverage my interdisciplinary background in applied and fundamental science to work as a R&D scientist in biotechnology or pharmaceutical companies. As a highly motivated individual, my goal is to make my own contribution in pushing the current therapeutic agents to the next level.

Chi (Aimee) Zhao, Ph.D.'s Current Company Details
10x Genomics

10X Genomics

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Automation Scientist | Biomedical Engineer
Chi (Aimee) Zhao, Ph.D. Work Experience Details
  • 10X Genomics
    Senior Scientist, Assay Automation
    10X Genomics Mar 2023 - Present
    Pleasanton, California, Us
  • 10X Genomics
    Scientist Ii, Asssay Automation
    10X Genomics Sep 2021 - Mar 2023
    Pleasanton, California, Us
  • Emerald Cloud Lab
    Scientific Developer
    Emerald Cloud Lab Feb 2020 - Aug 2021
    Austin, Texas, Us
    Develop automated workflow for ion chromatography and centrifuge experiments:1. Design experiment parameters in the codebase and automatically optimize parameter values based on various experiment setting.2. Interface with instrument software to facilitate efficient method file creation and data export during the experiments.3. Design and streamline experimental procedures to guide lab operators in performing experiments.4. Create qualification tests for instruments and the subsequent result reporting.5. Generate SOP for instrument maintenance.6. QA new functions and procedures prior to their official release.
  • The University Of Texas At Austin
    Graduate Research Assistant
    The University Of Texas At Austin Jan 2015 - Dec 2019
    Austin, Tx, Us
    1. Developing cell-derived biomaterials for specific drug delivery- Designed multifunctional transmembrane proteins that target cells on the basis of their surface receptor expression profiles. Further enhanced targeting selectivity by including serial repeats of the targeting ligand domain on the chimeric transmembrane proteins.- Genetically engineered donor cells to stably express multifunctional transmembrane proteins and harvested their plasma membranes as drug delivery vehicles.Cover photo: http://onlinelibrary.wiley.com/doi/10.1002/smll.201670136/fullPublication: http://onlinelibrary.wiley.com/doi/10.1002/smll.201600493/fullhttps://pubs.acs.org/doi/abs/10.1021/acs.biochem.7b006882. Engineering cell-derived biomaterials for efficient macromolecular delivery directly to the cytoplasm- Genetically engineered donor cells to stably express transmembrane channel proteins, connexin 43, providing macromolecules with a direct passageway into the cytoplasm- Designed and expressed a chimera version of connexin 43 protein with a RNA binding domain taken from virus in order to increase the concentration of siRNA in the cell-derived plasma membrane vesicles to enhance gene silencing- Quantitatively measure the copy number of siRNA in donor cells and cell-derived vesicles using qPCR3. Investigating the impact of receptor heterodimerization on the receptor uptake mechanism by endocytosis- Designed and constructed chimeric transmembrane model receptors in order to establish a simplified model system in live cells for this study- Used quantitative confocal imaging as well as publicly available MATLAB packages to map the distribution of receptors on the plasma membrane of cellsPublication: https://www.sciencedirect.com/science/article/pii/S0006349519305685https://pubs.rsc.org/am/content/articlelanding/2019/sm/c9sm00876d
  • Baylor College Of Medicine
    Undergraduate Researcher
    Baylor College Of Medicine Jan 2011 - Aug 2012
    Houston, Texas, Us
    Studied the structure of protein kinases activated by secondary messengers, specifically cyclic-GMP dependent protein kinase- Investigated the binding mechanisms of cGMP-dependent protein kinase to secondarymessengers, which is physiologically important in muscle relaxation and platelet activation- Purified, crystallized, and solved the structures of proteins in the cGMP dependent protein kinasefamily, such as cGMP-dependent protein kinase Iβ cyclic nucleotide binding domain (CNBD) B

Chi (Aimee) Zhao, Ph.D. Skills

Molecular Biology Data Analysis Microsoft Office Flow Cytometry Biomedical Engineering Public Speaking Cell Culture Molecular Cloning Fluorescence Microscopy Drug Delivery Statistics Real Time Polymerase Chain Reaction Western Blotting R Powerpoint Adobe Illustrator Mammalian Cell Culture Structural Biology Polymer Chemistry Translational Research Sirna Cell Engineering Protein Purification Biochemistry Cell Biology Biomaterials Gene Editing Protein Engineering Project Management

Chi (Aimee) Zhao, Ph.D. Education Details

  • The University Of Texas At Austin
    The University Of Texas At Austin
    Biomedical Engineering
  • Rice University
    Rice University
    Chemistry

Frequently Asked Questions about Chi (Aimee) Zhao, Ph.D.

What company does Chi (Aimee) Zhao, Ph.D. work for?

Chi (Aimee) Zhao, Ph.D. works for 10x Genomics

What is Chi (Aimee) Zhao, Ph.D.'s role at the current company?

Chi (Aimee) Zhao, Ph.D.'s current role is Automation Scientist | Biomedical Engineer.

What is Chi (Aimee) Zhao, Ph.D.'s email address?

Chi (Aimee) Zhao, Ph.D.'s email address is ch****@****xas.edu

What is Chi (Aimee) Zhao, Ph.D.'s direct phone number?

Chi (Aimee) Zhao, Ph.D.'s direct phone number is +171349*****

What schools did Chi (Aimee) Zhao, Ph.D. attend?

Chi (Aimee) Zhao, Ph.D. attended The University Of Texas At Austin, Rice University.

What are some of Chi (Aimee) Zhao, Ph.D.'s interests?

Chi (Aimee) Zhao, Ph.D. has interest in Health.

What skills is Chi (Aimee) Zhao, Ph.D. known for?

Chi (Aimee) Zhao, Ph.D. has skills like Molecular Biology, Data Analysis, Microsoft Office, Flow Cytometry, Biomedical Engineering, Public Speaking, Cell Culture, Molecular Cloning, Fluorescence Microscopy, Drug Delivery, Statistics, Real Time Polymerase Chain Reaction.

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