Review business strategy and patent. Design proof-of-concept experiment. Support in-lab equipment and experiment setup. Identify critical data for investment.

Extensive hands on platform development for personalized/precision medicine in cancer research.- Expert in Single-cell western blotting- Establishment of tissue culture facility in Herr Lab•Research Project: Application and characterization of single-cell western blottingo Application in breast cancer- Collaboration with Dr. Mark Pegram, Stanford School of Medicine, Stanford University. - Advance single-cell western blot with micro-gradient gel to allow assaying proteins with wide molecular weight range (e.g. 25-300 kDa) in a 1 mm short separation length. - Assess cell-to-cell variation of protein signaling in HER2+ breast cancer tissue. - Discern and quantify full length and truncated HER2 isoform in HER2+ breast cancer tissue. o Application in brain cancer - Collaboration with Dr. Sanjay Kumar, Depart of BioE, UC Berkeley.- Investigate the protein signaling pathway involved in glioblastoma cell invasion.- Build up an integrated in vitro platform of chemotaxis cell migration assay and single-cell western blot.o Application in brain cancer - Collaboration with Dr. Manish Aghi, Residence of Neurological Surgery, UCSF.- Identify and investigate the role of tumor-associated macrophage in Avastin resistance glioblastoma tissue. - Investigate the role of MIF (migration inhibitory factor) in Avastin induced resistance signaling.

•Research Project: Investigate the tumorigenesis process induced by low dose radiation in human mammary epithelial cells o Part of the “Low Dose Radiation Research Program” funded by U.S. Department of Energy (DOE) Scientific Focus Area (SFA) o Skilled in qRT-PCR (quantitative real time-PCR) to evaluate the RNA expression of cytokines o Established NF-kB-luc human mammary epithelial cell line and performed luciferase assay to evaluate the protein activation of NF-kB after stimulation. o Evaluated the inflammatory response, microenvironment and cell-cell communication effect in IR induced cytokine expression and NFkB signaling.

•Research Project: Investigate the molecular mechanism study of reversible and irreversible senescence in breast cancer cells o Awarded one-year funding (Apr 2011-Apr 2012) of “Postdoctoral Research Abroad Program” from Department of International Cooperation, National Science Council, Taiwan o Established Tet inducible breast cancer cell lines. o Measured cell cycle, cell proliferation and cellular senescence by PI staining, EDU, colony forming assay, SAHF (Senescence associated heterochromatin foci), β-galactosidase assay. o Skilled in immunoprecipitation, western blot and 2D gel to measure the protein expression and phosphorylation profile.

Structure Activity Relationship studies for fluorescence probes in cancer diagnosis. Develop and establish new methods for screening better cancer diagnosis probes.

Ph D. Thesis: BMVC related molecules in cancer research: cancer diagnosis and photodynamic therapy. Best Doctoral Thesis, “Novel Drug Development”, Chinese Chemical Society in Taiwan“Cancer cell detecting devices” (US Patent: 20070098233)“Fluorescent carbazole compounds for cancer diagnosis” (US Patent: 20110111429) -Characterized the cellular response of a small organic molecule, BMVC in lung carcinoma (H1299), lung adenocarcinoma (CL1-0) and normal lung fibroblast (MRC-5) cell lines.-Designed the staining protocol for applying BMVC in screening single cancer cells.-Developed a handheld device and flow system for utilizing BMVC in cancer diagnosis.-Collaborated with doctors in National Taiwan University for applying BMVC in screening head and neck tumors from fine needle aspiration samples, and tumors from pleural effusion.-Conducted a series of transport studies of BMVC in cancer cells.-Set up real time imaging system and procedures for evaluating the photodynamic therapeutic effect of o-2B-P in cancer cells.