Chih-Hsiang Lin Email & Phone Number

台灣 臺北市 南港區

BioTReC Nucleic Acid Pilot (BNAP) @ BioTRec, Academia SinicaConduct over 35 internal and external R&D project of RNA-LNP• Microfluidic and T-junction based encapsulation system• Finalize CPP of mRNA-LNP encapsulation for 3 customized special case(including LNP formulation with artificial lipid)• Evaluation of effect of feed pump on LNP quality of TFF system• Optimization of TFF process for 2 scaling up cases.• Automation of multi-channeled and manufacturing oriented TFF process• Automation of data processing for mRNA-LNP related analysis• Development of GMP compliance manufacturing

台灣 臺北市

• Standardize and document mRNA-LNP manufacturing• Fine tuning of critical process parameters (CPP) of encapsulation of mRNA-LNP• Initiate light scattering-based characterization of nanoparticle (DLS, AF4-MALS)• Initiate quantification of lipid component of mRNA-LNP (RP-UHPLC-CAD)• Optimize gradient-elution condition of RP-UHPLC for 3 different drug products• Optimize TFF of plasmid DNA• Assist in optimization of formulation of mRNA-LNP

台灣 台北市

Implemented production and purification of avidin-like proteinAnalyzed purified protein with electrophoresis and liquid chromatographyImplemented and optimized surface modification and conjugation of gold nanoparticleImplemented and optimized preparation of single component and phase separated supported lipid bilayerImplemented high speed imaging of nanoscale diffusion behavior of nanoparticleInitiated enzymatic biotinylation of membrane protein in living cell Outsourced on demand protein production and purification