Chris Nel, Phd Email and Phone Number

Lead internal CMC Process Development activities via hands-oncontribution in the laboratoryResponsible for drug substance of bioconjugates and biologics includingprocess development, tech transfer, scale-up, characterizationWork with CDMOs to manage external process development,optimization, and tech transfer of new programs in a stage appropriatemanner to ensure key milestones and products are delivered on schedulePartner with Research to support early stage bioconjugationdevelopment activitiesRepresent CMC and Process Sciences as a subject matter expert ininternal cross functional teamsContribute to preparation and review of CMC regulatory documentationfor biologic INDs and BLAs in support of relevant interactions with healthauthoritiesEstablish clear expectations and priorities, set objectives, and effectivelyapply resources to deliver on program goals

Contribute to set up a new oligonucleotide (sgRNA) process development laboratory and establishsupply chains, workflows and operation to model pmanufacturing processes.Analyze processes and manufacturing data to inform, plan and execute process development activities.Plan and execute process development studies (upstream/downstream) with the objective to introduceprocess improvements intro the GMP manufacturing of sgRNA. Support associated tech transfers.Prepare and perform solid phase oligonucleotide syntheses (scale-down model) and purifications aswell as associated in-process and final analytics as neededMaintain instrumentation, create/document laboratory processes. Generate reports and other technicaldocuments. Present context, data and finding to the team and cross functionally.Strategize with cross-functional transition teams (research development) and introduce newtechnologies to CMC workflowsAct as manufacturing SME for external mfg. campaigns and development projects interfacing withIntellia’s partnering CMOs and internal working groups (PM, EMSC, AD, QA/QC). Review processoutlines, batch records and manufacturing data.Access the literature, guidances for industry, databases, and technical documents to contribute toregulatory authoring and PD strategy.

• Synthesis of oligonucleotides, deprotection, purification, annealing and lyophilization of oligonucleotides to support all disease programs • Analytical characterization of the synthesized oligonucleotides for purity determination and impurity profiling• Support new chemistry discovery for the development of novel oligonucleotide conjugate platforms• Operate, maintain, and troubleshoot lab instruments such as oligonucleotide synthesizers, purification systems, HPLC and LC-MS• Conduct laboratory work in safe, efficient manner that ensures a safe working environment for colleagues • Ability to complete assignments and meet deadlines and time schedules• Keep accurate record of the experiments and summarize the results in written reports• Possess excellent written and verbal communication skills • Demonstrate an ability to work as part of a team and possess an excellent work ethic Ability to be successful in a fast-paced, intense work environment

Synthesis of biologically active aminoglycoside derivativesSolid phase peptide synthesis of peptide nucleic acids (PNAs) and peptidesAnalysis and purification of bioactive compounds via various techniques (column chromatography, prep HPLC (Agilent), analytical HPLC (Agilent), LC-MS (Waters), TLC, solution phase extractions, etc.)Development of LC-MS methodology to analyze peptides and PNAsTrain new postdoctoral hires in both solution phase and solid phase synthesis

Prepared and delivered recitation to 25 students in a non-majors laboratory course.Delivered recitation to General Chemistry I and II students.Maintained the grades of all students online.Prepared undergraduates with important safety information for the relevant lab course.Communicated complex topics to students in a way that allowed for open discussion and thought-provoking questions.Delivered problem sessions and lectures to 150+ organic chemistry students on a regular basis

Development and analysis of crosslinks between abasic (Ap) sites located within DNA.Development of studies to modulate Ap formation under various conditions. Synthesis of small molecules to mimic Ap sites within DNA.Generation of small library of biomimetic molecules design to study DNA damage and repair.Synthesis and purification of small drug molecules to be utilized in boron neutron capture therapy.Development and modification of hydrophilic polymers to enhance drug delivery.Formation of nanoparticles to be used as drug delivery vehicles.Maintenance of a BSL-2 biological lab, monitoring 9 various cancer cell lines.Oversaw development of three undergraduate students, aiding their acclamation to a research laboratory.Knowledge and previous usage of Gel Permeation Chromatography, 1H-NMR, 13C-NMR, including various 2D experiments, 10B-NMR, GC, HPLC, MS, and PAGE.Knowledge in analytical separations, including extractions, recrystallization, vacuum distillation, and column chromatography.

Led group sessions of introductory and organic chemistry students.Individually aided introductory and organic chemistry students in weekly problem sessions.Aided other tutors in teaching their students during the group sessions.