Christin Bauer Email & Phone Number

high content imagingassay development

Cambridge, United Kingdom

PROTACsprotein degradationlentiviral workcell based screening using high content imaging

Cambridge, England, United Kingdom

Glasgow, United Kingdom

https://www.nature.com/articles/s42255-020-0253-2?proof=tAnalysing the impact of hypoxia, various amino acid starvation and drug treatments using 12 different cancer cell lines in vitro. The aim was to better understand the role of Methionine salvage, polyamine synthesis and trans-sulfuration pathway. Also, I was responsible to set up various imaging protocols using the Operetta High-Content Imaging System from Perkin Elmer and to set up RNA extraction and qRT-PCR analysis within the lab.- Cell culture (various colon, breast, ovarian and pancreatic cancer cell lines)- High-content imaging (Operetta, Harmony, Columbus)- Western Blotting- RNA extraction, reverse transcription, qRT-PCR

Glasgow, United Kingdom

- Finishing my PhD work- First-author paper In Press in Cell Metabolism 'A neuronal relay mediates a nutrient responsive gut/fat body axis regulating energy homeostasis in adult Drosophila.'- Hands on experience with high content imaging and flow cytometry in addition to methods used during my PhD- written communication (manuscript writing)- oral communication (PhD defense, various public engagement events)

Glasgow, United Kingdom

- 4 years of analytical and solution-oriented metabolic research using a challenging animal model in international and interdisciplinary environment - Co-founder and organiser of professional development initiatives at our research institute; negotiated funding from industry partners and transformed students’ experiences - Uplifting team player with strong interpersonal and communication skills - written communication (writing PhD thesis)- oral communication (various scientific talks and public engagement events)- Enthusiastic about creating new methods and technologyMain techniques used: - Confocal microscopy- tissue dissections + immunofluorescence staining- RNA Isolation and quantification, cDNA synthesis, qRT-PCR- several metabolic readouts, using e.g. absorbance, fluorescence and luminescence assay kits- protein extraction and UV quantification, SDS-PAGE + Western Blotting- in vivo Time-lapse microscopy- extraction and characterization of mitochondria

Glasgow, United Kingdom

I got a Leonardo fellowship to fund a 6-month internship, which aimed to perform a RNAi screen of transcription factors affecting intestinal homeostasis in Drosophila melanogaster. Use of Drosophila genetics and confocal microscopy to analyse intestinal proliferation state in homeostatic and tissue-damaged conditions.

Master Of Science (M.Sc.), Molecular Genetics, 1.2

I have focused on the role of OCT4 in different colorectal cancer cell lines. Main techniques used: - cell culture - transfection methods.

Bachelor Of Science (B.Sc.), Genetics, 2.2

thesis title: „Etablierung von transgenen Drosophila-Linien zur Analyse von mRNA-trans-Spleißen“ (Establishing transgenic Drosophila lines.