Christina Chen Email and Phone Number

• Histology– Embedded 60 formalin-fixed tumor samples with paraffin. Prepared histologic slides using a Leica microtome.Performed both H&E staining and IHC staining for these specimens. Imaged them with the optical microscope. The results showed how human prostate cancer cell growth in mice.– Sectioned 98 formalin fixed paraffin embedded human bone samples with the microtome. Completed H&E staining and IHC staining, imaged all specimens, and documented them. Analyzed different types of bone metastasis due to prostate cancer based on the images.• Tissue Culture and Immunofluorescence– Studied human primary fibroblast calcium channels under the influence of cobalt ions using immunofluorescence. The results provided imaging evidence of mitochondria failure of the fibroblasts.– Learned q-PCR analysis of human primary fibroblasts.

My research included the following areas：• Designed a novel ammonia-induced mineralization coating on cell culture insert and characterized the coating• Developed protocol for osteoclast culture and resorption evaluation on ammonia-induced mineralization coating• Performed bisphosphonate delivery through ammonia-induced mineralized cell culture insert and studied its effects on osteoclastic resorption activity• Investigated the effect of prostate cancer cell-conditioned medium on osteoclastic resorption activity based on ammonia-induced mineralized cell culture insert• Systematically investigated the evaporation-induced surface crystallization for bio-mineralizationThis innovative work led to the creation of a robust bio-mineralized coating protocol. The versatility and reliability of this coating make it a valuable tool for osteoclastic resorption evaluation, anti-resorption drug delivery, and bone metastases study in vitro.

• Coordinated bi-weekly group meetings and individual meetings for the research group, fostering effective communication and collaboration.• Supervised monthly lab safety procedures and maintained records of the chemical inventory, ensuring a safe and compliant laboratory environment.• Coordinated activities for the Biomaterials Society BC Student Chapter in 2018, facilitating engagement and networking opportunities for chapter members.

Tutored MTRL 451 Microstructural Analysis Laboratory for 3 semesters: • Tutored a small group of undergraduate students on techniques of microstructural analysis, including specimen preparation, optical microscopy, X-ray diffraction, and scanning electronic microscopy.• Provided timely and frequent feedback to students; graded the analysis reports and final exams.

Throughout my Master's studies, I conducted a comprehensive investigation into the potential of electrospun titania fiber films as osteoinductive materials and assessed their biocompatibility with a hypotriploid human cell line.-Developed and characterized electrospun titania fiber films.-Systematically explored the biomineralization properties of electrospun titania fiber films.-Analyzed the results of protein resorption on electrospun titania fiber films.-Evaluated hypotriploid human cell proliferation on titania fiber films.