●Oversight of quality control in immunotherapy production laboratory ●Document control and product release ●Testing growth promotion of media, sterility of products ●Safety testing of biologics compounds ●Identification by microscopy of allergen raw materials (pollens, molds, animal dander) ●Compliance with USDA regulatory requirements acting as APHIS (Animal and Plant Health Inspection Service) liaison

●Sole scientist at a small biotech startup manufacturing regenerative medicine solutions for the veterinary market ●Processor of equine birth tissue-derived regenerative veterinary medicine products ●Shipping and supply chain coordinator ●Supervisor of charter school interns on student science projects ●Quality and Product Development manager

●Managed quality control for vitamin/nutritional supplements products ●Formulated new products and streamlined manufacturing of industrial hemp CBD products ●Expanded intellectual property protection and developed guidelines for GMP compliance

●Headed research and development laboratory, managed scientific staff, and directed existing and future product development ●Project manager for tissue processing and allograft production to improve existing products and manufacturing processes ●Promoted application of stem cell technology and explored new markets in regenerative medicine ●Projects: human bone putty formulation, amniotic fluid viscosupplement development and product launch, human extracellular matrix engineering for 3D microenvironment used in personalized medicine cancer diagnostic tests

●Taught two sections of laboratory for the General Biology course BIO181 for the Fall 2014 semester ●Administered course materials, conducted laboratory exercises, gave background lectures and proctored review sessions ●Prepared midterm and final exams ●Evaluated student performance in the classroom and through LoudCloud virtual classroom software portal

●Part-time position in Vitamin department at Sprouts Farmers Market, Ahwatukee (Phoenix) Arizona location ●Inventory maintenance: stocking shelves, rotating back stock onto shelves (FIFO), incorporating new products into sales inventory, setting up and breaking down displays, facing, handling product line resets ●Customer service: processing returns, special orders, holds, and rain checks, handling phone requests from customers, assisting customers in product selection, educating consumers on value of probiotics, omega-3 fatty acids, and immune support products ●Substituted as back door receiver reconciling $500K/month worth of inventory for the entire store

●Volunteer position in Department of Basic Medical Sciences, following poliovirus postdoctoral research ●Consultant to former supervisor in the shaping of laboratory results and data into a future publication ●Publication in preparation: Bradley CA, Hansen SJ, Hwang H, Polpitiya A, Liu J, Petritis K, and Gustin KE. Proteomic analysis reveals the consequences of poliovirus infection on nuclear pore complex composition.

●Postdoctoral research into poliovirus infection and modification of the host nuclear pore complex ●Protein purification: biochemical fractionation of nuclear envelope proteins ●Proteomics sample preparation and data analysis (Scaffold software, ExPASy on-line tools) ●Electrophoresis techniques including SDS-PAGE, immunoblotting, DNA electrophoresis ●Recombinant DNA techniques: primer design, polymerase chain reaction (PCR), subcloning ●Bioinformatics: Vector NTI software, on-line tools: GenBank, BLAST, PubMed, Gene Ontology ●Tissue culture: transient transfection, stable cell line selection, virology ●Microscopy: assay development with GFP (BiFC: bimolecular fluorescence complementation) ●Lab management: biological safety, chemical inventories, vendor interaction, cryostorage

●Postdoctoral research investigating the molecular basis of osteoarthritis and cell adhesion to extracellular matrix components. ●Recombinant protein: baculovirus/insect cell expression system, bacterial expression, purification by chromatography ●Radiation experience: metabolic protein labeling by [S-35] amino acid incorporation ●Regulatory duties: coordinator of Biological Use Authorization license, member Subcommittee for Research Safety, liaison with Health Physics and Nuclear Medicine departments, certified radioisotope user with Nuclear Regulatory Commission

●Postdoctoral research investigating role of Vitamin D in DNA repair pathways and head and neck cancers ●Lab management: purchasing decisions, setting up new lab equipment, protocol development, troubleshooting ●Proteomics: IEF, 2D gel electrophoresis, protein microarrays, Luminex bead-based multiplex assays ●Training: Taught laboratory techniques to graduate students, undergraduate student interns, and medical student

●Postdoctoral research investigating interactions between subunits of a protein synthesis holoenzyme complex (eIF3) and its involvement in regulation of eukaryotic protein synthesis initiation ●Biochemistry: mutagenesis by PCR, autoradiography, co-immunopurification ●Virology: verified, generated and amplified baculovirus stocks for multiple recombinant proteins ●Collaboration: reagent sharing with collaborating labs, managed Materials Transfer Agreement with technology transfer department and German cancer consortium (DKFZ)

●Graduate student research characterizing novel isoforms of housekeeping protein and proto-oncogene eIF4G-1 ●Laboratory: proteolysis, MALDI-TOF mass spectrometry, Southern blots, northern blots, primer extension analysis, splicing together bicistronic expression constructs ●Database management: EST (expressed sequence tag) database mining, assembly of exon-intron maps using draft genomic sequence, customizing Excel spreadsheets

●Managed quality control laboratory at chemical compounding facility ●Maintained compliance with environmental regulations for factory waste streams ●Interacted with sales force and customer service staff to provide technical support ●Handled hazardous materials and arranged hazardous waste disposal ●Trained laboratory and plant personnel in wet chemistry quality control testing ●Conducted OSHA safety training for factory employees