Christopher Chieco Email & Phone Number

Purification of harvested proteins and antibodies through the use of chromatography columns, ultra- and diafiltration, and viral filtration. Pooling, concentration, and dilution of eluates. Utilization and maintenance of AKTA Ready skids and Unicorn controller software. Qualification and resin packing of GE chromatography columns. Sampling for bioburden and endotoxin screening. pH and conductivity testing. Aseptic manipulations under a flow hood. Process filter integrity testing. Materials requisition, autoclaving, and kitting. Inventory management. Preparation of buffers. Working with clients to ensure understanding and product quality. Training of new manufacturing associates.

Durham, Nc

Routinely performing automated cell counts and micro/macroscopic observations on MRC-5 cells for monitoring growth and degeneration.Calibrating Vi-Cell automated cell counters daily.Calibrating and maintaining Guava easyCyte flow cytometers.Preparing samples for Guava assays and analyzing results.Aseptically diluting cell or stock virus seed solutions into cell growth media.Working slowly and deliberately in aseptic A (class 100) conditions.Carefully following SOPs and playbooks (process schedules) to ensure quality and efficiency.Operating and maintaining Robotic Cell Culture Systems via Delta-V.Using SAP to move and track goods and request work orders.Organizing and training individuals in Aseptic Behaviors according to Merck guidelines.Adhering to cGDP and GMP guidelines.Maintaining a 5S work environment.Training new employees in cGDP, GMP, and processes.Using PAS-X MES to follow a work flow and find information.Taking RODAC plate samples of technicians for environmental monitoring.Using BACH and HANDEL to log RODAC plates for environmental monitoring.Observing and reporting all safety issues, and following them up to ensure they are addressed.Maintaining a site-wide state of inspection-readiness for FDA, EU, and Asian inspections.

Analyzing samples using Cliquid and Analyst software.Reviewing and reprocessing chromatograph data.Maintaining, calibrating, tuning, and troubleshooting LC/MS instruments. OTS Extractions; Preparing reagentsMethod development for analyte extraction.Extracting samples to be analyzed by GC/MS, performing dilutions, hydrolysis, elutions, and derivitizations according to the SOP.Organizing and maintaining a clean, safe, and efficient lab environment.

During which time the restaurant became a management-training restaurant and a test kitchen for the 108 Carino’s restaurants worldwide. Working with staff members to ensure 100% guest satisfaction, as well as running the administrative office, which involved ordering goods, preparing deposits, and creating invoices. Teaching fledgling men and women how to be successful in a competitive environment.

Laying out pages using Quark and advising other writers for UNC’s satire magazine, which earned a nomination for the Edward Kidder Graham Outstanding Senior Award for public service.Editing copy for grammar and syntax. Making sure articles were there very best.Working with a variety of writers to make their ideas clearly and cohesively expressed in order to produce something great.

B.A., Biology/English

Activities and Societies: Independent Defense Council BoUNCe Magazine -- Associate Editor/Writer Aussie Rules Football Club Woody Allen.

Ba Biology, Biology, General