-Lead group of scientists focused on stable CHO engineering and process optimization.-Group supports the Discovery Biotherapeutic pipeline through CHO pool generation for pre-MCB, fed-batch analysis and scale up using single-use bioreactors.-Collaborate closely with Molecular Biology, Purification and Upstream Development groups.

-Mid to large scale protein production in 5 to 100L single-use bioreactors with DeltaV/Trubio.-Expressing multiple antibody-based architectures, native proteins, and Fc-fusions.-Early-stage candidate analysis and media development using DOE.

-Project Team Leader for internal rare disease glycoprotein drug candidate. -Managed departmental activities with Research Unit and Leadership.-Maintained satellite bioreactor operations for lab interfacing between Research and Development.

-Expression of new biological entities in CHO pools: cytokines, nanobodies, enzymes, and proteins of: human, mouse, rat, camel and shark origin.-Recombinant protein production supporting Discovery pipeline up to 100L in stainless-steel stir tank and wave bioreactors.

-Authored SOPs/MBRs. Led Tech transfer for VPRIV perfusion bioreactor clinical campaign -Setup new bioreactor lab for Cell Culture Process Development group

-Developed proprietary serum-free HT1080 media formulations for perfusion and fed-batch; used in Replegal and Idursulfase commercial manufacturing.-Evaluated perfusion devices: Acoustic Settler, TFF, External/Internal Spin filters-Operated 3-32L glass Braun Bioreactors

Dr.Frank Rogers Lab working for Dr Corinna Krinos investigating the pathogensis of Legionella pneumophila-Maintained Hybridoma cell lines. Purified monoclonal antibodies for In Vivo studies

Pathogenic Microbiology