Graduate Research Assistant
• Devised and executed synthetic routes, developing therapeutic molecules for the treatment of atopic dermatitis• Purified synthesized compounds using high-throughput separation techniques• Characterized synthesized compunds using various analytical techniques including MS and NMR• Successfully amplified and cloned gene sequence to express a recombinant fusion protein in DH5α and BL21 E. coli using traditional cohesive end cloning techniques• Optimized digestion and ligation conditions to improve cloning efficiency• Designed primers and optimized cycling conditions to validate positive clones using colony PCR