Graduate Research Assistant
- Devised and executed synthetic routes, developing therapeutic molecules for the treatment of atopic dermatitis
- Purified synthesized compounds using high-throughput separation techniques
- Characterized synthesized compunds using various analytical techniques including MS and NMR
- Successfully amplified and cloned gene sequence to express a recombinant fusion protein in DH5α and BL21 E. coli using traditional cohesive end cloning techniques
- Optimized digestion and ligation conditions to improve cloning efficiency
- Designed primers and optimized cycling conditions to validate positive clones using colony PCR