Christopher Parry Email & Phone Number

San Diego, California, Us

Senior Research Associate on the Process Development team tasked with designing, executing, and analyzing experiments to develop and refine the parameters between iPSC expansion,iCD34 differentiation, and iT differentiation for downstream product manufacturing of iPSC-derived T cell therapies.• Perform assessment of cell cultures, maintenance of iPSCs, hematopoietic differentiations, and T celldifferentiations.• Perform characterization of stage-specific processes utilizing flow cytometry, gene expressingprofiling, and media analysis.• Communication of culture assessments, results, and reagent inventories in a timely manner torelevant team members.

San Diego, California, Us

Senior Research Associate on the Cancer Immunotherapy team who worked closely with Principal Scientist to develop a unique proprietary iPSC derived off-the-shelf allogenic CAR T-cell targeted therapy.• Flow cytometry (CytoFLEX, Fortessa) panel design for testing iPSC derived CAR (T cells, NK cells) and cancer cells analyzing both surface & intracellular proteins.• Ex vivo: murine blood, liver, lung, spleen, & tumor processing for downstream analysis (flow cytometry) and presenting of results.• In vitro CAR T-cell clonal screening, co-culture/cytokine release assay (MSD), & ADCC to determine clonal efficacy.

San Diego, California, Us

Research Associate I & II on the Translational Sciences team, a team entrusted with the task of investigating and developing new immuno-oncological therapies for the photoimmunotherapy platform, Alluminox. Conquering Cancer.• Flow cytometry (CytoFLEX, Northern Lights) & panel design on immune (T cells, NK cells) and cancer cells analyzing surface & intracellular staining.• Ex vivo: tumor infiltrating lymphocyte & spleen processing, cell separation /flow cytometry, & pharmacokinetic assays: analyzing murine blood (AlphaLISA, ELISA, MSD) • Research & develop cytokines (IL-2, IL-12, & IL-15) and immunocytokines (superagonists) as cancer immunotherapies.• Contributed to IND filing; from performing experiments and writing about findings, to signing off for FDA submission• Perform routine lab safety walkthroughs, and offer lab safety guidance to team members as the Bio Safety Officer• Open and manage CRO accounts, i.e. off-site liquid nitrogen storage, laser instruments, outsourced experiments

San Diego, California, Us

• Responsible for the in vitro culture and expansion of cancer cells for in vivo preclinical mouse studies to support oncological research programs using photoimmunotherapy (PIT).• Perform in vitro PIT assays to test in house antibody conjugate potency and cell death• Maintain multiple cancer cell lines simultaneously, with scale-up performed as required for xenograft implants• Culture and expand hybridomas for sequencing, supernatant collection, and cryopreservation• Engineer new cell lines for use in in vitro and in vivo studies• Maintain tissue culture lab and equipment. Ordering stocking supplies as needed

Germantown, Maryland, Us

Research and development of two projects as part of the Human Therapeutics Division. 1) Exploring a potentially novel pathway to cure acute myeloid leukemia (AML) using exosomes as a targeting and delivery system to kill AML cells.2) Investigating and developing a repeatable pathway for reprogramming donor cord blood cells into induced pluripotent stem cells then differentiating iPSCs into patient derived T-cells. • Aseptic culturing of mammalian and primary cells for exosome production• Tangential flow filtration, Millipore Labscale TFF• Size-exclusion chromatography- IZON qEV, exosome purification• NanoSight nanoparticle tracking, exosome quantification• Characterizing exosomes by western blot and flow cytometry, looking for positive expression of tetraspanin proteins CD9, CD63, and CD81• Maintained a collection of iPSCs originating from donor cord blood cells for differentiation into T-cell like cells• Ran flow cytometry (iQUE) for QC of iPSC differentiation towards T-cells using hematopoietic stem cell (HSC) and common lymphoid progenitor (CLP) surface marker expression• Optimized a method of growing and passaging 3D iPSC suspension cultures

Waltham, Ma, Us

Investigating and developing an iPSC-derived in vitro disease model for long QT syndrome and other diseases. Utilized genome editing and iPSC differentiation to replicate disease phenotypes.• Aseptic culturing of mammalian and primary cells for disease models• Design and synthesize CRISPR gRNAs• Cloning and single-cell sorting, BD FACS Aria II• Collaborated with Rare Science and a child with a rare disease to investigate potential stem cell related cures for the disease. Developed a blueprint for a iPSC-derived disease model• Development of basic molecular biology lab skills – PCR, Western blot, gel electrophoresis, etc.

Bachelor’S Degree, Biotechnology

Occupational Safety And Health Technology/Technician