Christopher Parry Email & Phone Number

San Diego, California, US

• Senior Research Associate on the Process Development team tasked with designing, executing, and analyzing experiments to develop and refine the parameters between iPSC expansion,iCD34 differentiation, and iT.
• Perform assessment of cell cultures, maintenance of iPSCs, hematopoietic differentiations, and T celldifferentiations.
• Perform characterization of stage-specific processes utilizing flow cytometry, gene expressingprofiling, and media analysis.
• Communication of culture assessments, results, and reagent inventories in a timely manner torelevant team members.

San Diego, California, US

• Senior Research Associate on the Cancer Immunotherapy team who worked closely with Principal Scientist to develop a unique proprietary iPSC derived off-the-shelf allogenic CAR T-cell targeted therapy.
• Flow cytometry (CytoFLEX, Fortessa) panel design for testing iPSC derived CAR (T cells, NK cells) and cancer cells analyzing both surface & intracellular proteins.
• Ex vivo: murine blood, liver, lung, spleen, & tumor processing for downstream analysis (flow cytometry) and presenting of results.
• In vitro CAR T-cell clonal screening, co-culture/cytokine release assay (MSD), & ADCC to determine clonal efficacy.

San Diego, California, US

• Research Associate I & II on the Translational Sciences team, a team entrusted with the task of investigating and developing new immuno-oncological therapies for the photoimmunotherapy platform, Alluminox. Conquering.
• Flow cytometry (CytoFLEX, Northern Lights) & panel design on immune (T cells, NK cells) and cancer cells analyzing surface & intracellular staining.
• Ex vivo: tumor infiltrating lymphocyte & spleen processing, cell separation /flow cytometry, & pharmacokinetic assays: analyzing murine blood (AlphaLISA, ELISA, MSD)
• Research & develop cytokines (IL-2, IL-12, & IL-15) and immunocytokines (superagonists) as cancer immunotherapies.
• Contributed to IND filing; from performing experiments and writing about findings, to signing off for FDA submission
• Perform routine lab safety walkthroughs, and offer lab safety guidance to team members as the Bio Safety Officer

San Diego, California, US

• Responsible for the in vitro culture and expansion of cancer cells for in vivo preclinical mouse studies to support oncological research programs using photoimmunotherapy (PIT).
• Perform in vitro PIT assays to test in house antibody conjugate potency and cell death
• Maintain multiple cancer cell lines simultaneously, with scale-up performed as required for xenograft implants
• Culture and expand hybridomas for sequencing, supernatant collection, and cryopreservation
• Engineer new cell lines for use in in vitro and in vivo studies
• Maintain tissue culture lab and equipment. Ordering stocking supplies as needed

Germantown, Maryland, US

• Research and development of two projects as part of the Human Therapeutics Division. 1) Exploring a potentially novel pathway to cure acute myeloid leukemia (AML) using exosomes as a targeting and delivery system to.
• Aseptic culturing of mammalian and primary cells for exosome production
• Tangential flow filtration, Millipore Labscale TFF
• Size-exclusion chromatography- IZON qEV, exosome purification
• NanoSight nanoparticle tracking, exosome quantification
• Characterizing exosomes by western blot and flow cytometry, looking for positive expression of tetraspanin proteins CD9, CD63, and CD81

Waltham, MA, US

• Investigating and developing an iPSC-derived in vitro disease model for long QT syndrome and other diseases. Utilized genome editing and iPSC differentiation to replicate disease phenotypes.
• Aseptic culturing of mammalian and primary cells for disease models
• Design and synthesize CRISPR gRNAs
• Cloning and single-cell sorting, BD FACS Aria II
• Collaborated with Rare Science and a child with a rare disease to investigate potential stem cell related cures for the disease. Developed a blueprint for a iPSC-derived disease model
• Development of basic molecular biology lab skills – PCR, Western blot, gel electrophoresis, etc.

Bachelor’S Degree, Biotechnology

Occupational Safety And Health Technology/Technician