• Explored biophysical mechanisms of B. subtilis cell wall, cytoskeletal filaments MreB, FtsZ, and FtsA cell enzyme regulation to determine shape of peptidoglycan sacculus meshwork surrounding the bacterial cell• Under limited supervision used ensemble and single molecule microscopy to quantify active state of expressed proteins, input responses, and reactions to changed factors • Worked with plasmids, determining Culture Purity, Cell Density Determination, Endotoxin, Sterility, cell bank testing according to cGMP requirements • Troubleshoots issues related to equipment and assay performance and suggested improvements to lab protocols

• Profiled miRNA candidates in a high-throughput work environment with CSF samples from diseased model mice and samples from Mayo Alzheimer patients with qPCR, Western blotting, gel electrophoresis checking biomarker viability• Held accountable under minimal supervision for sample preparation, reagent preparation, equipment maintenance, tracking of laboratory consumables, assay development, QC activities, and data review • Working familiarity of immunological ligand binding assays and mouse pup brain sample extraction via self study and observation• Actively contributed to discussion of research papers in Mayo Clinic Neurobiology of Disease group lab meetings and seminar talks