Christopher Adase Email & Phone Number

San Diego, California, United States

• Method development and validation for Oncolytic virus, CAR-NK, and other programs: including NGS sequencing, troubleshooting, and method development; multi-color (>12 color) flow cytometry panel, design, development, and validation; qPCR assay development and cell based assays• Support CMC upstream and downstream groups for related projects• Support R&D to develop novel protein biomolecules and their subsequent purification, QC testing, and target effectiveness using internally developed methodologies

San Diego, California, United States

• Lead a small team focused on assay improvement of NA extraction improvement using a magnetic bead-based workflow, leading to a 10% yield increases from NA extractions, and ~15% decrease of hands-on time for extraction. Responsible for method transfer to CLIA laboratory, SOP and validation reports.• Lead researcher conducting pathfinding and validating experiments for novel skin cancer detection assays, evaluating new platforms for method development• Coordinate between Bioinformatics, and R&D Teams for experimental planning, validation, data analysis. Conduct literature searches into potential candidate genes and for other disease markers.

La Jolla

• Designed and produced multiple T cell stimulating vaccines and developed a rapidly customizable vaccine platform utilizing an adenovirus delivery system• Generation and clonal selection of stable cell lines used in production of therapeutic vaccines• Assay Development and construction of reporter cell lines for quality control assays• Create SOPs relating to quality control and release criteria of vaccine products• Developed methods to produce a novel STING pathway activator using engineered LMP1 fusion proteins

La Jolla Ca

Project 1: Identified and characterized effects of an antimicrobial peptide on growth factor expression in epidermal wounding• Identified novel changes in gene expression using NGS (RNA-seq) and subsequent analysis with PartekFlow with validation by qPCR, luminex multiplex ELISAs, IHC/Immunofluorescence, and siRNA knockdown in NHEKs, endothelial cells, and fibroblasts.• Subsequently, characterized affected signal transduction pathways (EGFR, etc.) through phosphoblotting and kinase inhibition• Measure changes in proliferation via colorimetric assays (ie. tetrazolium salt, MTT, SRB)Project 2: Characterized the role of IL-1R has in knockout mice predisposed to forming abnormal dermal growths• Identified and characterized a prominent pre-cancerous phenotype in UVB exposed mice with abrogated interleukin-1 receptor (IL-1R) signaling• Utilized RNA-seq comparison between wild-type and knockout tissue to generate pathway/gene leads further investigated via qPCR, sandwich ELISA, cell isolation and transformation, proliferation and apoptosis assays• Identified a critical role of IL-1R signaling in TNF release and cell death following UVB exposureProject 3: Pharmaceutical company funded research project• Identified novel changes in gene expression using RNA-seq with validation through ELISA, and taqman qPCR to determine effects of α-adrenergic-antagonists on cytokine production. Project results and reports led to a contract renewal for the project.• Involved in microbiome sample processing, histology, and staining of human clinical samples in a clinical trial determining skin barrier penetration by bacteria

La Jolla Ca

Project 1: Developed an ex vivo imaging assay to investigate gender-specific regulation of bulge stem cell proliferation in a murine model• Trailblazed and optimized conditions to maximize tissue survival and subsequent pharmaceutical compound screens to monitor changes in stem cell proliferation and/or migration.• Targeting HSP90 using mouse and human cell cultures, using tissue isolation and MACS cell sorting to enrich for stem cells with subsequent analysis by qPCR, IHC/IF, Chromatin Immunoprecipitation (ChIP).• Followed UCSD ACP guidelines and protocols for humane treatment of animals during mouse handling, breeding, genotyping, tissue dissection, and surgical procedures.Project 2: Novel epidermal differentiation and enhancer function using an activated BRAFV600E model• Analysis of p300 binding sites in skin from p300 ChIP-Seq data.• Target identification and cloning of 60+ enhancer elements bound by EP300 via gateway cloning.• Produced, isolated, and quantified lentivirus constructs followed by transfection of NHEKs.• Analysis of transfected cells through Fluorescent microscopy, Time-lapse microscopy, IHC/IF, and qPCR.• Utilized an in-house high throughput mouse model of Xeno-transplantation for screening, characterization, and analysis of previously identified enhancer constructs.

Taught courses every semester in a variety of biochemistry and genetics based courses and laboratories.Teaching responsibilities included creation and grading of exams, giving laboratory lectures, grading of lab reports and creating lecture content.Laboratory responsibilities included creation of chemical reagent stocks and setting up laboratories, performing, vetting and adjusting experiments as needed by the week prior to class, and the creation of stocks biological reagents.

Assisted professors, teaching assistants, or supervisors with preparation of laboratory materials and laboratory maintenance

Ph.D, Biochemistry & Biophysics

Activities and Societies: Gamma Sigma Delta, Biochemistry Graduate AssociationDissertation: The C-terminus of Transmembrane Helix 2 (TM2).

Master Of Science (M.Sc.), Biology

Thesis: Proteolytic activity in Arabidopsis thaliana during development Advisor: Dr. Susannah Gal; Binghamton Researched mRNA for multiple.

Bachelor Of Science (B.S.), Biochemistry

Activities and Societies: Washin Ryu Karate club