Chynna Broxton, Phd Email & Phone Number

Philadelphia, Pa, Us

Philadelphia, Pennsylvania, United States

• Develops qPCR assays to evaluate residual DNA size.• Develops cell- based assays to detect residual DNA transcription by qPCR and ddPCR technologies.• Responsible for authoring standard operating procedures (SOPS) and characterization memorandums to support IND Filings.• Performs routine qPCR testing to support process development.• Trained in GLP/GMP Compliance and Good Documentation practices.

Philadelphia, Pennsylvania, United States

• Performed Technical reviews for R&D and GMP Testing.• Developed custom Southern blot, Northern blot, and qPCR assays to determine transgene integration, transgene structural integrity, gene copy number, and RNA copy number.• Performed routine GMP testing to determine genetic stability across WCB, MCB and End- of-Production Cell lines.• Responsible for COT/COA report writing and release.• Participated in client communications on deliverables.• Responsible for scheduling GMP testing and allocating resources.

Philadelphia, Pa

• Characterized C. elegans and Zebrafish models of mitochondrial deficiency via PCR, gel electrophoresis, Western blot and Fluorescent Microscopy.• Performed Luminescence based cell viability assay using Primary Human Fibroblast Cells to test novel and standard of care therapies for mitochondrial disorders.• Developed protocols to monitor Zebrafish Larvae Swim Behavior Utilizing ZebraBox Viewpointsoftware.• Established a large particle flow cytometry assay to assess mitochondrial stress in C. elegans.• First Author of Published findings on the impact of standard of care therapies on C. elegans model of mitochondrial deficiency.

• Characterized the impacts of copper starvation on Superoxide Dismutase (SOD) enzymes role incellular signaling through gene expression analysis.• Performed RNA extraction and purification, cDNA synthesis, and qRT-PCR of antioxidant stress genesin Candida albicans (Broxton and Culotta, 2016).• Performed atomic absorption spectroscopy, fluorescence microscopy, and gel based enzymatic activity assays.• Experienced in bacterial and yeast cell culture.• Performed molecular cloning• Responsible for lab maintenance and reagent prep.• Authored 2 First- Author Publications and 1 Scientific Review Article

Lakewood, New Jersey

• Performed Quality control testing of finished pharmaceutical products for release and stability• Performed Organic Enzyme Extraction• Quantified Enzyme potency via UV-Vis and fluorescence spectrophotometry• Trained in GMP/GLP compliance and adherence to standard operating procedures (SOPs).

Doctor Of Philosophy - Phd, Biochemistry And Molecular Biology

Laboratory of Valeria Culotta Research focuses on the copper and manganese cytosolic superoxide dismutases of the pathogenic yeast Candida.

Bachelor'S Degree, Biochemistry

Activities and Societies: National Collegiate Honors Council