Clark Henderson work email
- Valid
Clark Henderson personal email
- Valid
- Valid
- Valid
My passion for learning and adventure has resulted in a professional career that has included a variety of experiences. Over the past 20 years I have worked in academia and biotech industry developing methodologies to investigate a variety of biomolecules to support everything from peer reviewed publications to IND enabling studies. My scientific expertise is dedicated to developing highly complex mass spectrometry assays.More specifically, my skills and experience include: • Over 15 years of experience with LC-MS analytical method development and validation, with over 10 years of experience in regulated laboratory environments (CLIA, CAP, GLP), for qualitative and quantitative analysis of proteins, peptides and small molecules in complex matrices (e.g. blood, plasma, urine, tissue) to support PK/PD and biomarker studies• Approximately 5 years of experience in method development and sample analysis using LC-MS based intact protein analysis to evaluate in vitro/in vivo biotransformations of antibody-drug conjugates (ADCs)• Managing regulated non-clinical and clinical outsourced studies ensuring strict adherence to regulatory guidance, as well as drafting documents for regulatory submissions • Management of multiple concurrent projects, including establishing and maintaining cross-functional collaborations with other scientists, as well as managing and mentoring junior scientists• Approximately 10 years of experience with discovery and targeted mass spectrometry method development and validation utilizing data dependent acquisition (DDA), data independent acquisition (DIA), parallel reaction monitoring (PRM) and multiple reaction monitoring (MRM), as well as troubleshooting, maintenance and repair of instruments • Experience utilizing statistical data analysis, modeling and interpretation in Excel and R, including Principal Component Analysis (PCA), Partial Least Squares-Regression (PLS-R), and hierarchal cluster analysis.I am always interested in meeting new people and learning about what they do, so please feel free to message me!
-
Associate DirectorSeagenBothell, Wa
-
Senior Principal ScientistPfizer Jul 2024 - PresentNew York, New York, Us -
Principal ScientistSeagen Jul 2022 - PresentBothell, Washington, Us
-
Senior ScientistSeagen Nov 2018 - PresentBothell, Washington, Us
-
Research ScientistUniversity Of Washington Jun 2017 - Nov 2018Seattle, Wa, UsResearch Scientist; May 2017 – PresentResearch scientist in Dr. Andrew Hoofnagle’s laboratory in the Department of Laboratory Medicine at the University of Washington Medical Center in Seattle, Washington• I have developed and collaborated with clinical chemists and medical laboratory scientists to validate an LC-MS-based method for absolute quantification of vitamin D binding protein (VDBP) in serum, including isoform identification, that has been utilized in over 10,000 samples by academic researchers as part of the University of Washington Research Testing Service; • I developed and am currently validating a shotgun proteomic assay for sub-typing amyloidosis in patient samples from FFPE tissue slides in collaboration with the MacCoss laboratory in the Department of Genome Sciences at the University of Washington. I am currently expanding methodology to utilize data independent acquisition (DIA) to replace data dependent approach;• I have developed and collaborated with clinical chemists and medical laboratory scientists to validate an LC-MS-based method for absolute quantitative analysis of retinol binding protein (RBP) in patient samples now currently offered in the Department of Laboratory Medicine at the University of Washington Medical Center which replaced an outdated clinical immunoassay. -
Senior FellowUniversity Of Washington Medical Center Apr 2013 - Jun 2017Seattle, Washington, UsSenior Fellow in Dr. Andrew Hoofnagle’s laboratory in the Department of Laboratory Medicine at the University of Washington Medical Center in Seattle, Washington• Developed and validated targeted proteomic method utilizing nano-liquid chromatography-parallel reaction monitoring-mass spectrometry for absolute quantification of hemoglobin A1c (HbA1c), apolipoprotein A-I (apoA-I) and apolipoprotein B (apoB) in dried blood spots;• Rapidly developed a targeted multiplexed urine proteomic assay using approximately 2000 samples for relative quantification of 20 proteins for a Juvenile Diabetes Research Foundation study to explore novel biomarkers for onset of diabetic kidney disease;• Developed a targeted multiplexed proteomic assay for relative quantification of 39 HDL-associated proteins in a cohort of patients from the Seattle Kidney Study to elucidate novel biomarkers for progression of diabetic kidney disease. -
Phd, Biophysics Graduate GroupUc Davis Sep 2007 - Apr 2013Davis, California, UsGraduate student researcher in Drs. Marjorie Longo and David Block’s laboratories in the Biophysics Graduate Group at the University of California, Davis•Coordinated and carried out experiments to understand how yeast cells adapt to different temperatures and increasing ethanol concentrations during fermentation by quantitatively analyzing their lipid composition utilizing LC-ESI-MS/MS and APCI-MS/MS, as well as measuring the membrane microstructure structure via fluorescence anisotropy and identifying intracellular metabolites using GC-MS and the Fiehn metabolomic library;•Designed and implemented anaerobic fermentation experiments, lipid extractions, and high-throughput quantitative LC-MS and FIA-MS analysis to determine the correlation between lipid compositional changes during alcoholic fermentation with yeast cell growth and ethanol production in 22 Saccharomyces cerevisiae strains using partial least squares (PLS) regression analysis to determine if lipids known to mitigate the toxic effects of ethanol in model membrane systems were correlated with higher final ethanol concentrations in yeast;•Developed normal phase HPLC-MS and APCI-MS (FIA-MS) analytical methods to rapidly and quantitatively analyze the phospholipid and sterol composition in fermenting Saccharomyces cerevisiae strains;•Prepared unilemellar and multilemellar liposomes and supported lipid bilayers to analyze and quantify membrane structural properties in a high ethanol environment utilizing epifluorescence and atomic force microscopy; •Carried out molecular model simulations of lipid bilayers composed of fluoridated phosphatidylcholines to elucidate mechanisms of lipid interdigitation utilizing GROMACS on a linux-based OS. -
Research AssociateJohns Hopkins School Of Medicine Dec 2005 - Jul 2007Baltimore, Md, UsResearch Associate in Dr. Christine Iacobuzio-Donahue’s laboratory in the Department of Gastrointestinal and Liver Pathology at The Johns Hopkins Medical Institute; Baltimore, Maryland•Assisted with tissue collection and carried out immunohistochemical (IHC) analysis to study E-cadherin expression and the mutational status of KRAS, TP53, and DPC4 as potential markers for grading the metastatic disease of pancreatic carcinomas;•Assisted with mammalian cell culture, colony formation, and MTT assays to analyze how up-regulation of NOTCH ligands are critical to Notch signaling in tumor initiation and maintenance in pancreatic neoplasms and how the use of γ-secretase inhibitors mitigated anchorage-independent growth in pancreatic cancer cell lines;•Assisted with RNA extractions, quantitative PCR and IHC experiments to analyze gene expression in normal, early- and late-stage primary pancreatic cancers and matched metastases to elucidate patterns of gene expression associated with advanced pancreatic cancer;•Began development of protocol utilizing capillary electrophoresis and TaqMan assays to rapidly detect loss of heterozygosity (LOH) in primary and metastatic tumors of pancreatic cancer;•Preliminary RNAi-mediated gene silencing experiments to facilitate protein expression in TP53 containing nonsense codon mutations by suppressing the expression of the hUpf protein complex and facilitate stop codon readthrough as assessed by western blot analysis;•Established method to detect circulating pancreatic tumor cells using magnetic based cell enrichment followed by flow cytometry analysis;•Developed primary pancreatic cancer cell lines using tissue obtained during post-mortem removal of primary and metastatic tumor tissue for xenotransplantation into mice;•Coordinated materials used in tissue sampling for the Gastrointestinal Rapid Medical Donation Program. -
Research AssociateUniversity Of Utah Sep 2002 - May 2005Salt Lake City, Utah, UsLaboratory technician in Dr. Raymond Gesteland and Dr. John Atkins’s laboratory in the Department of Human Genetics at the University of Utah; Salt Lake City, Utah•Designed and performed experiments to elucidate the change in frequency of +1 ribosomal frameshifting by substituting the native pseudoknot of the antizyme gene with antisense oligonucleotides to quantitate stop-codon readthrough in a cell free protein expression system via SDS-PAGE utilizing 35S-labelled methionine peptides and in mammalian cultured cells utilizing a dual luciferase reporter;•Performed experiments to explore the role of the programmed -1 ribosomal frameshift site in the SARS Corona Virus flanked by an upstream “slippery” heptanucleotide sequence and a putative downstream pseudoknot by cloning multiple pseudoknot constucts into a dual luciferase reporter system, followed by transfection into mammalian cells (HEK293) to assess dual-luciferase activity. Contructs containing GST and 6-His fusion proteins were expressed and purified to assess the frameshift product in HEK293 via ESI/MS;•Preliminary study to ascertain the efficacy of antisense RNA 2’-O-methyl oligonucleotides to stimulate nonsense codon mutation read-through in genes related to Duchenne Muscular Dystrophy;•Large scale transcription, translation, ribozyme cleavage, and purification of the Murine Leukemia Virus RNA pseudoknot for crystallization screens. -
Student Research AssistantWestminster College Sep 1999 - May 2001Salt Lake City, Utah, UsUndergraduate research assistant in Dr. Bonnie Baxter’s laboratory at Westminster College; Salt Lake City, Utah•Assisted in instructing students on the proper operation of laboratory instrumentation, performed literature searches, and designed equipment for the special needs of cultivating bacteria from high salt environments. -
Undergraduate ResearcherWestminster College Sep 1998 - Dec 1998Salt Lake City, Utah, UsParticipated in undergraduate research project under Dr. Judy Rogers at Westminster College; Salt Lake City•Responsibilities included extracting genomic DNA from Ground-Hornbill’s quills housed at the Tracy Aviary in Salt Lake City, Utah for phylogenetic study.
-
Student Research AssistantUniversity Of Utah Jun 1997 - Aug 1997Salt Lake City, Utah, UsUndergraduate research internship in Dr. Kent Ward’s laboratory in the Department of Human Genetics at University of Utah; Salt Lake City, Utah• Carried out PCR and data collection to screen patient DNA for pregnancy induced hypertension/preeclampsia study.
Clark Henderson Skills
Clark Henderson Education Details
-
University Of California, DavisBiophysics -
Westminster UniversityChemistry And Physics
Frequently Asked Questions about Clark Henderson
What company does Clark Henderson work for?
Clark Henderson works for Seagen
What is Clark Henderson's role at the current company?
Clark Henderson's current role is Associate Director.
What is Clark Henderson's email address?
Clark Henderson's email address is cl****@****ail.com
What schools did Clark Henderson attend?
Clark Henderson attended University Of California, Davis, Westminster University.
What are some of Clark Henderson's interests?
Clark Henderson has interest in Civil Rights And Social Action, Environment, Science And Technology, Disaster And Humanitarian Relief, Animal Welfare, Arts And Culture, Health.
What skills is Clark Henderson known for?
Clark Henderson has skills like Mass Spectrometry, Hplc, Chromatography, Biophysics, Sds Page, Cell Culture, Multivariate Statistics, Analytical Biochemistry, Immunoassays, Mammalian Cell Culture, Immunohistochemistry, Statistical Data Analysis.
Who are Clark Henderson's colleagues?
Clark Henderson's colleagues are Linda Dale, Nathalie Bruni, Pan Leung, Elise Cunningham, Jeff Pickering, Takimah Rein, Nike B..
Free Chrome Extension
Find emails, phones & company data instantly
Aero Online
Your AI prospecting assistant
Select data to include:
0 records × $0.02 per record
Download 750 million emails and 100 million phone numbers
Access emails and phone numbers of over 750 million business users. Instantly download verified profiles using 20+ filters, including location, job title, company, function, and industry.
Start your free trial