Clark Henderson Email & Phone Number

Bothell, Wa, Us

Bothell, Wa

New York, New York, Us

Bothell, Washington, Us

Bothell, Washington, Us

Seattle, Wa, Us

Research Scientist; May 2017 – PresentResearch scientist in Dr. Andrew Hoofnagle’s laboratory in the Department of Laboratory Medicine at the University of Washington Medical Center in Seattle, Washington• I have developed and collaborated with clinical chemists and medical laboratory scientists to validate an LC-MS-based method for absolute quantification of vitamin D binding protein (VDBP) in serum, including isoform identification, that has been utilized in over 10,000 samples by academic researchers as part of the University of Washington Research Testing Service; • I developed and am currently validating a shotgun proteomic assay for sub-typing amyloidosis in patient samples from FFPE tissue slides in collaboration with the MacCoss laboratory in the Department of Genome Sciences at the University of Washington. I am currently expanding methodology to utilize data independent acquisition (DIA) to replace data dependent approach;• I have developed and collaborated with clinical chemists and medical laboratory scientists to validate an LC-MS-based method for absolute quantitative analysis of retinol binding protein (RBP) in patient samples now currently offered in the Department of Laboratory Medicine at the University of Washington Medical Center which replaced an outdated clinical immunoassay.

Seattle, Washington, Us

Senior Fellow in Dr. Andrew Hoofnagle’s laboratory in the Department of Laboratory Medicine at the University of Washington Medical Center in Seattle, Washington• Developed and validated targeted proteomic method utilizing nano-liquid chromatography-parallel reaction monitoring-mass spectrometry for absolute quantification of hemoglobin A1c (HbA1c), apolipoprotein A-I (apoA-I) and apolipoprotein B (apoB) in dried blood spots;• Rapidly developed a targeted multiplexed urine proteomic assay using approximately 2000 samples for relative quantification of 20 proteins for a Juvenile Diabetes Research Foundation study to explore novel biomarkers for onset of diabetic kidney disease;• Developed a targeted multiplexed proteomic assay for relative quantification of 39 HDL-associated proteins in a cohort of patients from the Seattle Kidney Study to elucidate novel biomarkers for progression of diabetic kidney disease.

Davis, California, Us

Graduate student researcher in Drs. Marjorie Longo and David Block’s laboratories in the Biophysics Graduate Group at the University of California, Davis•Coordinated and carried out experiments to understand how yeast cells adapt to different temperatures and increasing ethanol concentrations during fermentation by quantitatively analyzing their lipid composition utilizing LC-ESI-MS/MS and APCI-MS/MS, as well as measuring the membrane microstructure structure via fluorescence anisotropy and identifying intracellular metabolites using GC-MS and the Fiehn metabolomic library;•Designed and implemented anaerobic fermentation experiments, lipid extractions, and high-throughput quantitative LC-MS and FIA-MS analysis to determine the correlation between lipid compositional changes during alcoholic fermentation with yeast cell growth and ethanol production in 22 Saccharomyces cerevisiae strains using partial least squares (PLS) regression analysis to determine if lipids known to mitigate the toxic effects of ethanol in model membrane systems were correlated with higher final ethanol concentrations in yeast;•Developed normal phase HPLC-MS and APCI-MS (FIA-MS) analytical methods to rapidly and quantitatively analyze the phospholipid and sterol composition in fermenting Saccharomyces cerevisiae strains;•Prepared unilemellar and multilemellar liposomes and supported lipid bilayers to analyze and quantify membrane structural properties in a high ethanol environment utilizing epifluorescence and atomic force microscopy; •Carried out molecular model simulations of lipid bilayers composed of fluoridated phosphatidylcholines to elucidate mechanisms of lipid interdigitation utilizing GROMACS on a linux-based OS.

Baltimore, Md, Us

Research Associate in Dr. Christine Iacobuzio-Donahue’s laboratory in the Department of Gastrointestinal and Liver Pathology at The Johns Hopkins Medical Institute; Baltimore, Maryland•Assisted with tissue collection and carried out immunohistochemical (IHC) analysis to study E-cadherin expression and the mutational status of KRAS, TP53, and DPC4 as potential markers for grading the metastatic disease of pancreatic carcinomas;•Assisted with mammalian cell culture, colony formation, and MTT assays to analyze how up-regulation of NOTCH ligands are critical to Notch signaling in tumor initiation and maintenance in pancreatic neoplasms and how the use of γ-secretase inhibitors mitigated anchorage-independent growth in pancreatic cancer cell lines;•Assisted with RNA extractions, quantitative PCR and IHC experiments to analyze gene expression in normal, early- and late-stage primary pancreatic cancers and matched metastases to elucidate patterns of gene expression associated with advanced pancreatic cancer;•Began development of protocol utilizing capillary electrophoresis and TaqMan assays to rapidly detect loss of heterozygosity (LOH) in primary and metastatic tumors of pancreatic cancer;•Preliminary RNAi-mediated gene silencing experiments to facilitate protein expression in TP53 containing nonsense codon mutations by suppressing the expression of the hUpf protein complex and facilitate stop codon readthrough as assessed by western blot analysis;•Established method to detect circulating pancreatic tumor cells using magnetic based cell enrichment followed by flow cytometry analysis;•Developed primary pancreatic cancer cell lines using tissue obtained during post-mortem removal of primary and metastatic tumor tissue for xenotransplantation into mice;•Coordinated materials used in tissue sampling for the Gastrointestinal Rapid Medical Donation Program.

Salt Lake City, Utah, Us

Laboratory technician in Dr. Raymond Gesteland and Dr. John Atkins’s laboratory in the Department of Human Genetics at the University of Utah; Salt Lake City, Utah•Designed and performed experiments to elucidate the change in frequency of +1 ribosomal frameshifting by substituting the native pseudoknot of the antizyme gene with antisense oligonucleotides to quantitate stop-codon readthrough in a cell free protein expression system via SDS-PAGE utilizing 35S-labelled methionine peptides and in mammalian cultured cells utilizing a dual luciferase reporter;•Performed experiments to explore the role of the programmed -1 ribosomal frameshift site in the SARS Corona Virus flanked by an upstream “slippery” heptanucleotide sequence and a putative downstream pseudoknot by cloning multiple pseudoknot constucts into a dual luciferase reporter system, followed by transfection into mammalian cells (HEK293) to assess dual-luciferase activity. Contructs containing GST and 6-His fusion proteins were expressed and purified to assess the frameshift product in HEK293 via ESI/MS;•Preliminary study to ascertain the efficacy of antisense RNA 2’-O-methyl oligonucleotides to stimulate nonsense codon mutation read-through in genes related to Duchenne Muscular Dystrophy;•Large scale transcription, translation, ribozyme cleavage, and purification of the Murine Leukemia Virus RNA pseudoknot for crystallization screens.

Salt Lake City, Utah, Us

Undergraduate research assistant in Dr. Bonnie Baxter’s laboratory at Westminster College; Salt Lake City, Utah•Assisted in instructing students on the proper operation of laboratory instrumentation, performed literature searches, and designed equipment for the special needs of cultivating bacteria from high salt environments.

Salt Lake City, Utah, Us

Participated in undergraduate research project under Dr. Judy Rogers at Westminster College; Salt Lake City•Responsibilities included extracting genomic DNA from Ground-Hornbill’s quills housed at the Tracy Aviary in Salt Lake City, Utah for phylogenetic study.

Salt Lake City, Utah, Us

Undergraduate research internship in Dr. Kent Ward’s laboratory in the Department of Human Genetics at University of Utah; Salt Lake City, Utah• Carried out PCR and data collection to screen patient DNA for pregnancy induced hypertension/preeclampsia study.

Phd, Biophysics

Bachelor Of Science (Bs), Chemistry And Physics