Carmen Montoya Gutiérrez Email & Phone Number

Copenhague, Región Capital, Dinamarca

Upstream Scientist in Mammalian team for Commercial Production. Responsibilities of the job: -Support to the upstream process during production -Preparation and updating of Master Production Records-Handling of deviations, CAPAs and change requests. -Improving production processes continuously-Interaction with different stakeholders (process development, manufacturing, quality departments)

Copenhagen, Capital Region, Denmark

Special course & Master Thesis in the Mammalian Cell Line and Bioprocess Engineering Group. The project's aim was to study in depth the role of signal peptides in the secretion pathway in Chinese Hamster Ovary cells. The productivity of three monoclonal antibodies was evaluated from the screening of different signal peptide combinations. For that, I designed and performed a high-throughput cloning experiment to generate a large set of plasmid vectors. I later evaluated the cell productivity in a high-throughput transient transfection experiment in CHO cells. From the results, relevant features of both signal peptides and antibodies were analyzed. Skills acquired: design of high-throughput experiments, optimization of cloning methods, skilled in different cloning techniques (USER cloning, NEBuilder, PCR mutagenesis) for vector construction, restriction enzyme digestion, media preparation, bacterial culture, DNA extraction methods, whole plasmid sequencingMammalian cell culture: sterile technique, cell counting, cell imaging (Celligo), transfection, transient evaluation, flow cytometry and FACS sorting, liquid handler experience (E2P2), measurement of antibody production with Octet biosensor, generation of stable cell lines through RMCE, junction PCR, qPCR.Statistical analysis: JMP, Minitab, Excel, Graphpad Prism.Literature review and presentation of results.

I conducted my project at the Bacterial Synthetic Bacterial Group under Felipe Gonzalo Tueros and Morten Sommer supervision. The aim of this project was to identify the reason behind the loss of productivity of L-DOPA producing plasmids in Eschericia coli Nissle. From a previous evolution experiment, plasmids from different timepoints were extracted. The genes enconding for the synthesis pathway of L-DOPA were PCR amplified and sent for Sanger Sequencing. Appearance and accumulation of mutations were studied and a timeline was established. Correlations between mutations and productivity were determined. Moreover, other laboratory techniques were performed as a further training experience. The main purpose of this project was to become comfortable with routine molecular biology lab work and developing research skills. Lab skills acquired: plasmid extraction, PCR, electrophoresis, Sanger Sequencing, bioinformatic analysis, CRISPR-Cas9, electroporation,

Mendavia, Comunidad Foral De Navarra, España

I was in charge of performing the laboratory tasks upon harvested grape arrival as well as the fermentation process during the harvesting season of 2020. Some of those tasks included: o Quality control of the harvested grapes o Analysis of wine tanks o Monitoring of the fermentation process o Quality control of bottled wine.

Msc In Eng. In Biotechnology, Biological/Biosystems Engineering

Intern at the Bacterial Syntethic Biology Group (The Novo Nordisk Center for Biosustainability) Intern at the Mammalian Cell Line.

Biology

I completed my Bachelor Degree in Biology in 2020. I conducted my Bachelor Thesis titled "Prime Editing: a new promising tool for human.

Corso Di Laurea, Scienze Biologiche

Study stay in Florence as part of the Erasmus+ programme (2018/2019). I had the opportunity to explore topics in which I had special.