Signed an offer in September, and Moved across the country to start at a new role on the molecular biology and gene editing team, was affected by the mass layoff In Jan 2023.

-Created a core like service of full suite cell line engineering pipeline for CRISPR knockout and LV based overexpression----Individually Created about 45 research cell lines in multiple cell types, evenly divided between Crispr knockout and LV overexpression ----Process was full scale from design of vectors and sgRNA to FACS validation and or sorting through freezer and inventory management----Listed as an inventor on a company patent for a novel RMCE cell line generated for improving the thorough put of screening----Developed multiple improvements based on literature research and course work education to improve the throughput and stability of generated research lines ----Served as local subject matter expert in cell line engineering offering assistance and support to team members----Presented this data to multiple levels of management on a regular basis-Supported Vector design team goals with molecular biology skill suite----Generated about 250ml of 2nd and 3rd generation lentiviral material per week in adherent 293 and shake flask LV max, including concentration and inventory management for animal studies and basic research----Transduced and performed copy number DDPCR assay on a variety of cell types for evaluating the efficacy of changed----Regularly performed 3-4 color FACS experimental design, execution and data analysis on team samples as well as trained new members on those skills-Built an in house custom 3d printing service----Designed and deployed custom lab quality of life inventions, including custom tube racks, pipette holders, animal study device mounts, centrifugation adapters freezer boxes and dividers

-Responsible for medium scale, Cell based Bio-Manufacture and harvest of proprietary gene therapy vector----Key member of a small production team with numerous concurrent projects and deadlines, involving plasmid DNA transfection, viral transduction, cell culture and DNA analysis----Managed tightly regulated lab notebook and responsible for maintaining various lab supplies----Common tasks included working with 10 Liter batch cultures of custom SF9 cells, performing daily transfections and transductions and then large batches of DNA preps each week. Success was measured in purity of final product as well as high volume production. ----Worked on a team of 5 people and regularly collaborated and exchanged work

Worked as an intern for 1 year post college on multiple different teams-Developed a reporter assay to evaluate protein expression in a disease relevant cell line ----Designed and optimized a standard protocol for plating cells and accurately reading the reporter assay in a 384 well format based on successful FACS results----Operated and designed protocols as needed for several screening/liquid handing technologies including Agilent Bravo, BIO MEK, Thermofisher Multidrop, HP liquid printer, SpectraMax m5 and High throughput imager -Assisted CRISPR screening approaches----Maintained multiple iPSC derived cell types in necessary state of differentiation, including all neural progenitor cells for the assay design team----Performed viral purification and transduction, genomic DNA and RNA purification, qPCR, FACS, and antibody staining to produce and evaluate expression changes-Managed protein expression analysis for a site directed mutagenesis project ----managed HEK cell line and performed E. coli transformation, protein lysis, SDS PAGE and western blots, followed by expression data analysis

-Optimized CRISPR/Cas9 experiment genome editing efficiency in disease relevant primary cell line----Participated in the design and optimization of gRNA and Cas9 selection, independently performed editing by electroporation and measured outcomes by TIDE analysis----Presented research findings to all research staff as a self imposed internship capstone----Received year long extension based on good performance