Colleen Richards Email and Phone Number

• Utilized regulatory and industry guidance to develop cell therapy workflows for translation to GMP manufacturing• Established batch records, bill of materials, capacity planning, and pricing for a wide range of cell therapy projects• Optimized cell culture, modification, expansion, and evaluation processes for a variety of applications• Collaborated with internal and external partners on projects including viral and non-viral modification,reprogramming, differentiation, large-scale expansion, and characterization• Co-founded activities committee for the entire site to provide educational, team-building, and charitable events for all coworkers to promote professional and personal development

• Collaborated with internal and external partners to develop cell therapy workflows, focusing on CAR-T products• Established processes for immune cell isolation processes (T-Cell, NK cell) and expansion in small- and large-scale vessels• Optimized modification processes including viral (lentivirus, AAV) transduction and non-viral (CRISPR/Cas9) transfection• Performed characterization via flow cytometry and image analysis

• Established cell culture, expansion, transfection, and characterization processes for the large-scale production of gene therapies• Lead the creation of electronic batch records and transition to Master Control system for data collection in accordance with ISO 9001 standards• Coordinated with business operations teams to implement SmartSheets for project scheduling and capacity planning• Collaborate with downstream colleagues to set up processes for downstream purification of viral vectors using chromatography (affinity and AEX) and TFF

• Developed workflows and analysis methods for Sendai virus and episomal reprogramming as well as CRISPR/Cas9 gene editing of human cells• Investigated the impact of several small molecules on knock-out and knock-in efficiencies• Optimized gene editing processes through the modification of guide RNAs and knock-in DNA sequences• Utilized flow cytometry, antibody staining, cell imaging, and PCR-based methods to quantify gene editing and reprogramming efficiencies• Cultured cells including cell lines, primary fibroblasts, primary PBMCs and T-cells, and iPSCs

• Led bioprinter setup and functionality testing in collaboration with Advanced Solutions Life Sciences.• Completed research 3D bioprinting of a cell-laden hydrogel construct to be used in transplantation studies• Developed sample preparation and crosslinking methods for a Pluronic F127-alginate hydrogel bioink• Optimized printing of the hydrogel constructs with live fibroblasts, set up culture conditions post-printing, and established methods for evaluation of viability and expansion

• Effectively communicated with participants regarding the outdoor equipment available for rental, specifically what would be most appropriate for their needs by providing general outdoors advice and Poly Escapes trip information• Encouraged a love of the outdoors and promoted access to outdoor activities for all Cal Poly students• Consistently enforced Cal Poly ASI and Poly Escapes rental policies regarding liability, safety, payment, and fees • Checked in participants for the rock climbing wall and ensured that all were informed of safe use policies• Operated a point-of-sales system for daily sales, rentals, registrations, and policy-related fees

• Managed and maintained day-to-day operations for Poly Escapes at Cal Poly, including operation of the rock climbing wall, trips program, and rental center• Supervised, scheduled, and maintained job and safety training for a staff of 25-30 undergraduate and graduate student employees.• Conducted quarterly performance evaluations and then worked with employees throughout the year on areas in which they needed improvement• Performed recruitment and training tasks when hiring new staff members and assisted in planning all-staff training for 250+ employees as well as staff appreciation events• Regularly evaluated rental and climbing equipment for safety and functionality and planned an annual sale for items which were no longer fit for rental but still functional

• Worked with the Advanced Materials Science team on the testing and analysis of polymeric membranes to be used in sensor design for continuous glucose level monitoring• Established methods for materials testing, data analysis, test method verification and validation, and documentation of results in accordance with regulatory guidelines• Developed skills in working with a range of materials analysis techniques, including tensile testing, gel permeation chromatography (GPC), Rheometry, and Fourier-transform infrared spectroscopy (FTIR)

• Created and executed test protocols for the characterization of materials currently in use by Stryker Neurovascular (project details protected by NDA)• Collected data, analyzed it, and then organized it into a database format for future use• Collaborated with industry professionals, professors, and fellow students to ensure project success

• Worked on a team of 3 students to design a microfluidic pump for use in an on-campus laboratory• Designed a gravity-fed system to allow for media addition during the incubation of cells on a microfluidic chip within a humidified incubator• Performed an in-depth prototyping process using the machine shops on campus and utilized the same shops to manufacture the final product• Collaborated with professors and students from other laboratories to perform microfluidic testing of the final product to ensure that the flow characteristics met the requirements given by the lab

• Was responsible for the approximately 3,000 women who were members of Panhellenic sororities at Cal Poly, for the 11 chapters on campus, and for the 10 members of the Panhellenic Executive Board• Served as a liaison for the Panhellenic community to campus and community organizations as well as to the National Panhellenic Conference and the national headquarters of each sorority on campus• Work with on-campus organizations included the Dean of Students’ office, the Office of Student Affairs, and Associated Students Incorporated. •Off-campus, worked with the San Luis Obispo City Council, Police Department, and Student Community Liaison Committee. • Presided over weekly General Panhellenic and Executive Board meetings and met regularly with the Fraternity and Sorority Life advisors and chapter presidents to continuously improve the operations of the organizations that our councils oversee• Created new educational programs for up to 3,000 students related to diversity and inclusion, education, and service, and effectively led other students in their various roles

• Worked with 9 other members of the Top Executive Board to ensure that all Alpha Phi International and Cal Poly Panhellenic standards were being met• Planned weekly events for 250+ chapter members with a budget of about $10,000• Established processes for educating all chapter members on the history of Alpha Phi and its values through education sessions and targeted programming events• Successfully carried out the chapter initiation ceremony for the 91 new members of Fall Quarter 2018• Guided and worked with the 8 Executive Directors under my position in the Programming and Education department to accomplish all of these things

• Was responsible for holding over 250 sorority members accountable for attaining satisfactory grades and recording all members' GPAs at the end of each quarter to report to Cal Poly Panhellenic• Created a new system of efficient data collection using resources already available at Cal Poly • Encouraged a high standard of academic excellence within Alpha Phi by offering academic resources and educating members on chapter standards• Met with members who were struggling academically and created comprehensive academic success plans, then checked in with them periodically to ensure that they were following the set plans

• Worked closely with other Junior Board members, the Executive Panhellenic Board, the Inter-fraternity Council, United Sorority and Fraternity Council, ASI, and other on and off campus organizations• Planned events on campus, assisted with philanthropy drives, and collaborated with other organizations listed above• Collaborated most closely with the Vice President of Panhellenic to promote scholarship on the Cal Poly campus, perform administrative duties, and plan and record relevant meetings and events