Constance Musseau Email & Phone Number

Enkhuizen, North Holland, Netherlands

Uppsala, Comté D'Uppsala, Suède

Postdoctoral researcher in Charles Melnyk groupPlant Biology Department - SLUPlant grafting is an ancestral technic, consisting of joining two plants to create one chimeric plant. However, the molecular mechanisms of grafting capacities in plants remain poorly understood. In this context, my research project aims at identifying genes involved in graft formation in plants, using Arabidopsis thaliana and tomato plant models.Main achievements:- Generation of transgenic material in Arabidopsis thaliana and tomato- In-depth phenotyping characterization of mutant lines, presenting defects in regeneration- Protocol optimization for micrografting of Solanaceae species, and in different temperature conditions. This work was part of a project published in Development in 2022.- Protocol optimization for monocots grafting, in collaboration with the University of Cambridge. This work led to a publication in Nature in 2021.

Région De Bordeaux, France

Fruit tissue morphology is a key character defining the final fruit quality. However, the cellular and molecular mechanisms by which fruit tissue morphology is defined remain incomplete. In this context, the aim of my thesis was to identify and characterize new regulators of fruit tissue morphology. Through a forward genetic strategy, I investigated the role of a Guanylate Binding Protein (GBP) protein at the origin of a thin pericarp phenotype in tomatoes. I studied in parallel the role of the protein in tomato and Arabidopsis thaliana models. My work suggests a role for the GBP in the fine control of cell division in the tomato pericarp, and was published in Plant Cell in 2020.Main achievements:- Mapping-by-sequencing strategy (according to Garcia et al., 2016)- DNAseq analyses- In-depth phenotyping characterization of tomato EMS mutant- Generation of Arabidopsis thaliana mutant lines using CRISPR-Cas9- Generation and maintenance of transgenic BY-2 tobacco cells- Tissue preparation for RMN and LC-MS analyses

Villenave D'Ornon - France

Phenotypic characterization (at the fruit, tissue, cellular and molecular levels) of tomato EMS mutants presenting alterations in fruit growth and endoreduplication. This work led to the publication of an article in Frontiers in Plant Science in 2017.Main achievements:-Detailed phenotyping characterization of tomato EMS mutants at the plant, cellular and molecular levels-Flux-cytometry analyses

Aix-En-Provence, Provence-Alpes-Côte D'Azur, France

Internship in Laboratory of Plant Development in CEA Cadarache, supervised by Dr. Benjamin PéretCharacterization of LPR1 gene, which is involved in phosphate starvation response in Arabidopsis thaliana roots.Main expertise- Plastic imbedding of Arabidopsis thaliana seedlings- Expression analyses

Villenave D'Ornon

Internship in "Plant Biology and Pathology" unit, in "Cherry Adaptation of Climate Change" team.Phenotypic and molecular characterization of cherry hybrids

Doctor Of Philosophy - Phd, Plant Biology

International Master "Plant Biology And Biotechnology", Biologie Végétale

Licence Biologie- Santé, Biologie Moléculaire, Cellulaire Et Physiologie